利用分子标记对辣椒抗马铃薯Y病毒的3个QTLs进行选择

为获得含有特定基因型的个体，深入研究辣椒抗马铃薯Y病毒（PVY）不同QTLs之间的关系，利用AFLP、SCAR和CAPS标记在辣椒材料‘Perennial’和‘Yolo Wonder’组成的BC₂和BC₁S₁分离世代对抗PVY的3个QTLs位点进行了选择。结果从BC₂群体中选出47个在主效QTL上含有抗性位点的单株，从中选出25个在LG9 PY2染色体上含有抗性位点的单株，最后选出11个在LG11 P10染色体上含有抗性位点的单株；从BC₁S₁群体中选出55个在LG9 PY2染色体上具有纯合抗性位点的单株，从中选出12个在主效QTL上纯合抗性位点的单株。经卡平方检测，实际分离比例同理论分离比例差异不显著。

Molecular Markers Assisted Selection for Three QTLs Resistant to PVY in Pepper(Capsicum annuum L.)

Markers assisted selection is still seldom used in pepper breeding in China, though many markers are reported recently. In order to obtain genotypes for researching of relationship of QTLs resistant to PVY, AFLP, CAPS, SCAR markers were used to assist selection of QTLs for resistance to PVY in BC₂ and BC₁S₁ segregating progenies from pepper 'perennial' and 'Yolo Wonder'. With the markers, 47 plants with resistant allele in major QTL locus, then from which 25 plants with resistant allele in LG9 PY2, at last 11 plants with resistant allele in LG11 P10 were selected from the BC₂ population. And 55 plants with homozygous resistant allele in LG9 PY2, and 12 plants with homozygous resistant allele in major QTL were selected from the BC₁S₁ population. X² analysis showed that the segregating ratio in fact is not significantly different with that in theory.