| 农杆菌介导大豆未成熟子叶的遗传转化 |
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| 引用本文： | 王萍,王罡,季静,周智明,郭威,拉巴,吴颖. 农杆菌介导大豆未成熟子叶的遗传转化[J]. 大豆科学, 2004, 23(2): 86-90 |
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| 作者姓名： | 王萍 王罡 季静 周智明 郭威 拉巴 吴颖 |
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| 作者单位： | 解放军军需大学植物基因工程研究中心,长春,130062 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 用农杆菌LBA4404(pGBI121S4ABC)转化5个大豆品种的未成熟子叶,经卡那霉素筛选得到抗性植株,进一步用PCR检测,获得10株PCR阳性植株.同时发现,不同大豆品种农杆菌转化的效率是不同的,吉林43较易于转化;共培养时间是影响转化效率的主要因子.
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| 关 键 词： | 大豆(Glycine max) 农杆菌 遗传转化 PCR检测 |
| 文章编号： | 1000-9841(2004)02-0086-05 |
GENETIC TRANSFORMATION OF IMMATURE COTYLEDON VIA AGROBACTERIUM TUMEFACIENS IN SOYBEAN |
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| Wang Ping Wang Gang Ji Jing Zhou Zhiming Gui Wei La Ba Wu Ying. GENETIC TRANSFORMATION OF IMMATURE COTYLEDON VIA AGROBACTERIUM TUMEFACIENS IN SOYBEAN[J]. Soybean Science, 2004, 23(2): 86-90 |
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| Authors: | Wang Ping Wang Gang Ji Jing Zhou Zhiming Gui Wei La Ba Wu Ying |
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| Abstract: | Immature cotyledon of 5 different soybean varieties were transformed by Agrobacterium tumefaciens LBA4404(pGBI121S4ABC) and resistant plants to kanamycin were obtained. 10 egenerated plants gave positive PCR reaction. Transformation efficiency was different among 5 varieties. Jilin 43 was easy for transforming by Agrobacterium tumefaciens. The time of co-cultivation is one of the most important factors for transformation efficiency. |
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| Keywords: | Soybean (Glycine max) Agrobacterium tumefaciens Genetic transformation PCR detection |
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