| 150个小麦品种（系）抗叶锈基因Lr35分子鉴定 |
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| 引用本文： | 魏新燕,杨文香,刘大群,孔俊英. 150个小麦品种（系）抗叶锈基因Lr35分子鉴定[J]. 中国农业科学, 2004, 37(12): 1951-1954 |
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| 作者姓名： | 魏新燕 杨文香 刘大群 孔俊英 |
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| 作者单位： | 河北农业大学植物保护学院,保定,071001 |
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| 基金项目： | 国家自然基金项目资助(30170602) |
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| 摘 要： | 小麦抗叶锈基因Lr35是成株抗性基因,在二叶期即表现连续抗性,被认为是有用的抗病资源。本研究利用以PCR为基础的STS、SCAR分子标记技术,对150个小麦品种(系)进行了分析,检测出8个小麦品种(6068,白蚰包,中麦9,早洋,碧玛1号,小偃7631,东方红3号和Madsen)含有Lr35基因。结合室内接种鉴定技术 (叶锈菌株为对Lr35无毒力的99-8-11-5),结果表明,这8个小麦品种虽在分子水平上含有Lr35基因,但在侵染类型上具有一定差异,其中东方红3号表现对菌株的亲和性。
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| 关 键 词： | 小麦叶锈病 Lr35 分子标记检测 STS SCAR |
| 收稿时间： | 2003-05-26 |
MAS for Leaf Rust Resistance Gene Lr35 in 150 Wheat Cultivars |
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| WEI Xin-yan,YANG Wen-xiang,LIU Da-qun,KONG Jun-ying. MAS for Leaf Rust Resistance Gene Lr35 in 150 Wheat Cultivars[J]. Scientia Agricultura Sinica, 2004, 37(12): 1951-1954 |
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| Authors: | WEI Xin-yan YANG Wen-xiang LIU Da-qun KONG Jun-ying |
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| Abstract: | The objective of this work was to identify the wheat cultivars containing the adult plant leaf rust resistance gene Lr35 by PCR-STS markers and PCR-SCAR markers. Out of 150 cultivars, 8 cultivars (6068, Baiyoubao, Zhongmai 9, Zaoyang , Bima 1, Xiaoyan 7631, Dongfanghong 3, Madsen) were identified to contain the Lr35 gene. In greenhouse experiment, the 8 cultivars were inoculated with an avirulent race 99-8-11-5 at the second-leaf stage, and they showed a resistant reaction as the known cultivars containing Lr35, except for Dongfanghong 3 showing higher infection type . |
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| Keywords: | Wheat leaf rust Lr35 Marker-assisted selection STS SCAR |
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