| 甘肃省中西部小麦白粉菌生理小种RAPD分析 |
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| 引用本文： | 甘丽萍,王生荣. 甘肃省中西部小麦白粉菌生理小种RAPD分析[J]. 河南农业科学, 2009, 0(2) |
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| 作者姓名： | 甘丽萍 王生荣 |
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| 作者单位： | 1. 重庆三峡学院,生物系,重庆,万州,404000;甘肃农业大学,草业学院,甘肃,兰州,730070
2. 甘肃农业大学,草业学院,甘肃,兰州,730070 |
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| 基金项目： | 甘肃省中青年科技基金 |
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| 摘 要： | 利用RAPD标记对采自甘肃省中西部20个小麦白粉菌菌株进行了研究。结果表明:在用9个扩增结果比较明显的随机引物扩增得到的81个RAPD位点中,有54个具多态性,多态性位点频率(单卫星方法统计)为66.7%。根据PopGene 32软件计算的Shannon多样性指数与Nei基因指数分别为0.455 6和0.296 8,多态性频率为96.3%,说明白粉菌菌株间具有丰富的遗传多样性。聚类分析结果表明,多态性特征与菌株地理来源之间存在一定的相关性,与生理小种毒性关系不显著。
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| 关 键 词： | 小麦白粉菌 生理小种 随机扩增多态性DNA 多态性频率 |
RAPD Analysis of Wheat Powdery Mildew(Blumeria graminis f. sp. tritici) in the Mid-west of Gansu Province |
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| GAN Li-ping,WANG Sheng-rong. RAPD Analysis of Wheat Powdery Mildew(Blumeria graminis f. sp. tritici) in the Mid-west of Gansu Province[J]. Journal of Henan Agricultural Sciences, 2009, 0(2) |
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| Authors: | GAN Li-ping WANG Sheng-rong |
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| Affiliation: | 1.Biological Department;Chongqing Three Gorges University;Wanzhou 404000;China;2.College of Lawn Science;Gansu Agricultural University;Lanzhou 730070;China |
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| Abstract: | Polymorphism of 20 isolates of Blumeria graminis f.sp.tritici collected from Mid-west of Gansu province was studied with RAPD.PCR amplication with 9 primers for the polymorphic frequency of 54 polymorphic loci showed 81 loci and the polymorphic frequency were 66.7%.PopGene 32 of Shannon and Nei were 0.4556 and 0.2968 respectively with polymorphic frequence 96.3%,which indicated there had high heredity differentiation among isolates.Clustering analysis showed that polymorphic characteristic was related to ge... |
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| Keywords: | Blumeria graminis f.sp.tritici Physiologic race RAPD Polymorphic frequence |
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