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半夏遗传结构和多样性RAPD标记分析
引用本文:张君毅. 半夏遗传结构和多样性RAPD标记分析[J]. 中国农学通报, 2010, 26(18): 43-47
作者姓名:张君毅
作者单位:华侨大学,福建厦门,361021
基金项目:华侨大学人才引进项目"半夏传多样性研究",福建省自然科学基金项目"短葶山麦冬遗传多样性评价及质量控制" 
摘    要:利用RAPD标记技术对取自半夏主要分布区16个省的24份种质进行遗传多样性分析。结果表明:在物种水平上,多态位点百分率(PPL)68.77%,Nei’S基因多样性指数(He)0.2062;居群水平上,PPL为9.88%~58.02%,平均27.21%,群体间遗传分化系数(GST)0.5282,居群间基因流(Nm)0.4466。UPGMA聚类分析显示,当相似系数0.896时,24份种质可分为5个类群。

关 键 词:福美双  福美双  戊唑醇  小麦赤霉病  复配  细胞膜透性  内含物  
收稿时间:2010-06-29
修稿时间:2010-07-15

Genetic Structure and Diversity among Pinellia ternata Revealed by RAPD Markers
Zhang Junyi. Genetic Structure and Diversity among Pinellia ternata Revealed by RAPD Markers[J]. Chinese Agricultural Science Bulletin, 2010, 26(18): 43-47
Authors:Zhang Junyi
Affiliation:Zhang Junyi (Huaqiao University, Xiamen Fujian 361021)
Abstract:To protect and utilize germplasm resources ofPinellia ternate , random amplification of polymorphic DNA (RAPD) marker was occupied to study the genetic diversity of 24 germplasm from 16 main distribution provinces. The results showed that at species level polymorphic loci percentage (PPL) is 68.77%, Nei’s gene diversity index (He) 0.2062. At population level, PPL varied from 9.88% to 58.02%, with the average value 27.21% and groups of genetic differentiation factor (Gst) is 0.5282, population gene flow (Nm) 0.4466. The results of cluster analysis based on UPGMA revealed that when similar coefficient was 0.896, 24 populations could be divided into five material groups.
Keywords:Pinellia ternata  random amplification of polymorphic DNA (RAPD) marker  genetic diversity  genetic structure
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