| 鹅细小病毒VP3基因重组禽痘病毒的构建和表达 |
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| 引用本文： | 赵丽荣,王君伟,贾永清. 鹅细小病毒VP3基因重组禽痘病毒的构建和表达[J]. 中国预防兽医学报, 2003, 25(6): 437-439 |
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| 作者姓名： | 赵丽荣 王君伟 贾永清 |
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| 作者单位： | 东北农业大学,动物医学院,黑龙江,哈尔滨,150030 |
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| 基金项目： | 黑龙江省“十五”攻关课题 (合同编号 :GB0 1B5 0 3_0 2 ) |
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| 摘 要： | 本实验采用质脂体转染方法将禽痘病毒转移载体质粒PSY681VP3LacZ转染被禽痘病毒FPV-017感染的鸡胚成纤维细胞CEF，通过蓝白筛选和6轮蚀斑克隆纯化，获得了稳定的重组病毒。PCR鉴定，重组病毒基因组中含有VP3基因。Dot-ELISA实验证明，重组病毒表达了VP3，并具有抗原性。
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| 关 键 词： | 鹅细小病毒 VP3 重组禽痘病毒 |
| 文章编号： | 1008-0589(2003)06-0437-03 |
| 修稿时间： | 2003-01-21 |
Construction and expression of recombinant fowlpox virus of goose parvovirus VP3 gene |
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| ZHAO Li-rong,WANG Jun-wei,JIA Yong-qing. Construction and expression of recombinant fowlpox virus of goose parvovirus VP3 gene[J]. Chinese Journal of Preventive Veterinary Medicine, 2003, 25(6): 437-439 |
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| Authors: | ZHAO Li-rong WANG Jun-wei JIA Yong-qing |
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| Abstract: | By lipofectamine chicken embryo fibroblast(CEF) cell cultures which were infected with FPV-017 were transfected with the complex of PSY681VP3LacZ plasmid and liposomal.Recombinant FPV-017 was selected and purified 6 times in CEF cell cultures with agar containing X-gal through blue plaques.The recombinant was identified by PCR.It revealed that VP3 gene was inserted into FPV-017.The expression of VP3 gene and its immunogenicitv were proved by Dot-ELISA. |
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| Keywords: | GPV VP3 recombinant fowlpox virus |
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