Screening methods and identification of QTLs associated with late maturity α-amylase in wheat |
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Authors: | Kolumbina Mrva Daryl Mares |
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Institution: | (1) Department of Plant Science, University of Adelaide, Waite Campus, Glen Osmond, SA, 5064, Australia |
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Abstract: | The synthesis of high levels of germination-type (high pI isozymes) α-amylase was induced in wheat genotypes prone to late
maturityα-amylase (LMA) following the exposure of detached tillers to cool temperature during grain development. The detached
tiller method was successfully applied to a range of genotypes and to a doubled haploid (DH) population derived from the cross
Cranbrook (LMA genotype) × Halberd (low amylase). The number of grains in ripe, treated tillers that contained high pI α-amylase
isozymes was measured using an ELISA antibody kit highly specific for high pI isozymes. Quantitative trait loci (QTL) controlling
the expression of LMA in wheat were detected in DH population Cranbrook × Halberd. The DH population and parents were sown
in 2 replicated sowings at the same location with sowing times differing by 2 weeks. QTL analyses were conducted separately
for each sowing, but results from both sowings were consistent and indicated a highly significant (it p > 0.01) QTL on the long arm of chromosome7B, with Cranbrook contributing the higher value allele. A second QTL with less
significant effect was found on the long arm of chromosome 3B, on the basis of data from the first sowing.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | antibody (ELISA) kit detached tillers late maturity α -amylase QTL Triticum aestivum (L em Thell) |
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