| 转hFat-1基因猪外源基因整合分析及拷贝数测定 |
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| 引用本文： | 华文君,华再东,郑新民,毕延震,肖红卫,张立苹.转hFat-1基因猪外源基因整合分析及拷贝数测定[J].江西农业学报,2014(11):99-101. |
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| 作者姓名： | 华文君 华再东 郑新民 毕延震 肖红卫 张立苹 |
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| 作者单位： | 湖北省农业科学院 畜牧兽医研究所,湖北 武汉,430064 |
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| 基金项目： | 转基因生物新品种培育重大专项(2011ZX08010-3、2014ZX08006-003)；国家自然科学基金项目(31201790)；湖北省农业科技创新中心项目(2011-620-001-003)。 |
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| 摘 要： | 对转人源化Fat-1基因猪的15头传代仔猪进行PCR检测,发现有7头阳性猪,其断奶后成活5头仔猪。对这5头仔猪进行Southern印迹杂交试验,确认3头仔猪整合有hFat-1基因。采用荧光实时定量PCR法测定原代转hFat-1基因公猪及3头阳性仔猪外源基因的拷贝数,分别是2、1、1、2个拷贝。转hFat-1基因猪能够将外源基因遗传到下代仔猪,但外源基因具有遗传不稳定的趋向。
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| 关 键 词： | 猪 转hFat-1基因 整合 拷贝数 |
Analysis of Exogenous Gene Integration and Copy Number in hFat-1 Transgenic Pigs |
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| HUA Wen-jun,HUA Zai-dong,ZHENG Xin-min,BI Yan-zhen,XIAO Hong-wei,ZHANG Li-ping.Analysis of Exogenous Gene Integration and Copy Number in hFat-1 Transgenic Pigs[J].Acta Agriculturae Jiangxi,2014(11):99-101. |
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| Authors: | HUA Wen-jun HUA Zai-dong ZHENG Xin-min BI Yan-zhen XIAO Hong-wei ZHANG Li-ping |
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| Institution: | (Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China) |
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| Abstract: | Fifteen heads of hFat-1 transgenic offspring piglets were detected by PCR, 7 heads of piglets were positive, and 5 heads of piglets survived after weaning.Southern blotting cross experiment confirmed that the exogenous gene hFat-1 had been integrated into 3 piglets among these 5 piglets.The copy numbers of hFat-1 in primary hFat-1 transgenic boar and 3 positive piglets were detected by fluorescence real-time Q-PCR, and they were 2, 1, 1, and 2 copies respectively.The integration of exogenous gene in hFat-1 transgenic pigs could be inherited to F1 -generation piglets, but could not be stably inherited. |
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| Keywords: | Pig Transgene hFat-1 Integration Copy number |
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