| 威宁绵羊IGF-1基因外显子多态性分析 |
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| 引用本文： | 刘标,易鸣,张依裕,王振,程朝友,杨德文,程均华,付正仙,王进. 威宁绵羊IGF-1基因外显子多态性分析[J]. 山地农业生物学报, 2017, 0(2): 82-85. DOI: 10.15958/j.cnki.sdnyswxb.2017.02.018 |
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| 作者姓名： | 刘标 易鸣 张依裕 王振 程朝友 杨德文 程均华 付正仙 王进 |
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| 作者单位： | 1. 贵州大学动物科学学院/贵州大学高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳,550025;2. 贵州省威宁县畜禽品种改良站,贵州威宁,553100 |
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| 基金项目： | 2013年地方畜禽品种保护项目贵州省农委 |
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| 摘 要： | 为了更好地保护和开发利用威宁绵羊这一优良地方品种,本实验利用威宁绵羊构建DNA池,设计4对引物扩增威宁绵羊IGF-1基因外显子及部分内含子序列。将PCR产物纯化并进行双向测序,通过DNAStar生物软件分析确定多态性位点。利用在线生物信息学软件分析多态位点对IGF-1基因RNA的二级结构、类胰岛素生长因子1的二级和三级结构的影响。结果表明,在扩增的IGF-1基因中筛选到3个SNPs:Intron1-A4387C、Exon2-T87C、Exon4-A27T,其中Exon4-A27T为错义突变,导致编码的精氨酸(Arg)变为丝氨酸(Ser);Exon2-T87C多态位点均未改变氨基酸的编码,为同义突变;Intron1-A4387C在内含子区,不参与氨基酸编码。
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| 关 键 词： | 威宁绵羊 IGF-1基因 SNPs |
Detection of Polymorphism of IGF-1 Gene in Weining Sheep |
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| LIU Biao,YI Ming,ZHANG Yi-yu,WANG Zhen,CHENG Chao-you,YANG De-wen,CHENG Jun-hua,FU Zheng-xian,WANG Jin. Detection of Polymorphism of IGF-1 Gene in Weining Sheep[J]. Journal of Mountain Agriculture & Biology, 2017, 0(2): 82-85. DOI: 10.15958/j.cnki.sdnyswxb.2017.02.018 |
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| Authors: | LIU Biao YI Ming ZHANG Yi-yu WANG Zhen CHENG Chao-you YANG De-wen CHENG Jun-hua FU Zheng-xian WANG Jin |
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| Abstract: | In order to protect and utilize the fine local variety,DNA pool of Weining sheep was constructed.4 primers were designed to amplify exon and intron sequences of IGF-1 gene in Weining sheep.Products of PCR were purified and bidirectional sequenced to determine the SNPs.Influence of polymorphic sites on the RNA secondary and tertiary structure of IGF-1 gene was analyzed by online bioinformatics software.The result showed that 3 SNPs were detected in IGF-1 gene,namely Exon2-T87C,a synonymous mutation;Exon4-A27T,a missense mutation which changed the code of Arg into Ser;and Intron1-A4387C,a noncoding mutation in the intron. |
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| Keywords: | Weining sheep IGF-1 gene SNPs |
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