Identification of weakly beta-hemolytic porcine spirochetes by biochemical reactions, PCR-based restriction fragment length polymorphism analysis and species-specific PCR |
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Authors: | Ohya Tatsuo Araki Hiroshi Sueyoshi Masuo |
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Institution: | Kyushu Research Station, Environmental/Enzootic Diseases Research Team, National Institute of Animal Health, Chuzan, Kagoshima, Japan. ohyat@affrc.go.jp |
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Abstract: | We examined the usefulness of PCR-based restriction fragment length polymorphism (PCR-RFLP) and species-specific PCR combined with a newly devised rapid biochemical test using microplates for identifying weakly beta-hemolytic intestinal spirochetes (WBHIS) isolated from pigs. WBHIS strains showing atypical biochemical characteristics were decisively identified at the species level by PCR-RFLP and species-specific PCR. Identification of WBHIS at the species level in routine diagnostic work will certainly contribute to clarifying the pathogenicity of WBHIS. |
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