富含硫氨基酸转基因大豆对根际土壤微生物群落结构的影响

选用两组转基因品质改良大豆（富含硫氨基酸）为材料，利用磷脂脂肪酸（PLFA）法分析了在大田种植条件下，两组转基因大豆对土壤微生物群落结构的影响。结果表明，与非转基因大豆相比，转基因品种土壤微生物群落的结构发生明显的变化。在两组转基因品种中PLFA组分均发生变化，其中转基因品种A组中占主导地位的14种分别是是革兰氏阳性菌（GP）的特征脂肪酸16:0、10Me18:0和好氧细菌特征脂肪酸18:1ω7c，Ｂ组中占主导地位的13种是表征GP的脂肪酸15:0、16:0和非特征脂肪酸14Me16:0。在A组的OE-8品种及B组的17-4和57品种中，表征细菌的PLFA含量和总PLFA含量与非转基因品种相比有显著上升。结果表明种植转基因大豆影响根际土壤微生物群落结构。

Effect of transgenic soybean with sulfur-rich amino acids on soil microbial population structure

Effects of transgenic soybeans on microbial population structure of rhizosphere soil were investigated under field conditions based on phospholipid fatty acid(PLFA) method.Two groups of transgenic soybeans with sulfur-rich amino acids were used.Results showed that soil microbial population in rhizosphere of transgenic lines changed significantly in both group A and B.15 specific PLFAs were identified in group A,and 13 in group B.Correlation analysis found that microbial characterization of group A and the total concentration of PLFA’s of the transgenic line OE-8 were significantly higher than non-transgenic line,and so were 17-4 line and 57 line in group B.The dominant fifteen PLFA mown plots were 16∶ 0,10Me18∶ 0 and 18∶ 1ω7c in group A,and dominant thirteen PLFA were 15∶ 0,16∶ 0 and 14Me16∶ 0 in group B.The results suggested that the microbial population structure changed after planting transgenic soybeans.