| 茶树CsPLt基因的荧光定量PCR分析 |
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| 引用本文： | 陈林波,夏丽飞,梁名志,江昌俊. 茶树CsPLt基因的荧光定量PCR分析[J]. 西南农业学报, 2011, 24(6) |
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| 作者姓名： | 陈林波 夏丽飞 梁名志 江昌俊 |
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| 作者单位： | 1. 云南省农业科学院茶叶研究所,云南勐海,666201
2. 安徽农业大学教育部茶叶生物化学与生物技术重点开放实验室,安徽合肥,230036 |
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| 基金项目： | 国家自然科学基金(30871568) |
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| 摘 要： |
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| 关 键 词： | 茶树 多元醇转运子 qRT-PCR 表达分析 |
Expression Analysis of CsPLt Gene in Tea Plant [Camellia sinensis(L) O.Kuntze] by qRT-PCR |
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| CHEN Lin-bo , XIA Li-fei , LIANG Ming-zhi , JIANG Chang-jun. Expression Analysis of CsPLt Gene in Tea Plant [Camellia sinensis(L) O.Kuntze] by qRT-PCR[J]. Southwest China Journal of Agricultural Sciences, 2011, 24(6) |
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| Authors: | CHEN Lin-bo XIA Li-fei LIANG Ming-zhi JIANG Chang-jun |
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| Affiliation: | CHEN Lin-bo1,XIA Li-fei1,LIANG Ming-zhi1,JIANG Chang-jun2(1.Tea Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Menghai 666201,China,2.Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of Education,Anhui Agricultural University,Anhui Hefei 230036,China) |
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| Abstract: | The cDNA-AFLP was applied to identify genes expressed differentially in cold acclimatized tea plant.A cDNA fragment,TDF6 encoding a polyol transporter was isolated.The cDNA fragment had 77 %,76 %,75 %homology with polyol transporter genes from Populus trichocarpa,Hevea brasiliensis and Lotus japonicus by BLAST on NCBI,and named CsPLt.qRT-PCR analysis showed that CsPLt gene were up-regulated by cold,dehydration,NaCl,the maximum expression were 7.3,12.2,5.3 times higher than that before treatment,respectively... |
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| Keywords: | Camellia sinensis Polyol transporter qRT-PCR Expression analysis |
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