Isolation of genes expressed during compatible interactions between powdery mildew (Blumeria graminis) and wheat |
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| Authors: | Li Zheng Junbin Huang Dazhao Yu |
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| Affiliation: | aDepartment of Plant Protection, College of Plant Science and Technology, The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China;bFaculty of Environmental Science and Engineering, Guangdong University of Technology, Guangdong, Guangzhou 510006, China;cPlant Protection Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China |
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| Abstract: | Powdery mildew of wheat is an important disease caused by the obligately biotrophic fungus Blumeria graminis. In compatible interactions, the powdery mildew fungus undergoes a series of developmental stages to form haustoria within host cells through which nutrients are obtained. In this study, we utilized the cDNA-AFLP technique to isolate wheat genes expressed at 5 days' post-inoculation. The expression patterns of several sequences identified in the cDNA-AFLP profiling were further investigated by Northern hybridization and RT-PCR analyses. Genes with sequence similarity to GenBank accessions AAR91119, P20076, BT009372, ABA99697, BAD81963 or AAO72574 showed greater expression in susceptible rather than resistant or mock-inoculated leaves. In addition, several sequences with similarity to CAD27894, XM_466672, AAT79487 or AAM64566 were expressed only in the compatible interactions. Possible involvement of these genes in susceptibility of host wheat upon powdery mildew infection is further discussed. |
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| Keywords: | cDNA-AFLP Powdery mildew Wheat Compatibility |
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