利用淘汰奶牛卵巢生产体外受精胚胎

以淘汰奶牛卵巢为材料,研究了卵巢采集方法、卵丘细胞和颗粒细胞对卵母细胞体外受精后发育的影响。结果表明奶牛屠宰后取其卵巢,用剖解法(平均9.1枚/卵巢),可比抽吸法(平均6.5枚/卵巢)得到更多的可用卵母细胞(p<0.01)。周围无卵丘细胞包围的裸露卵母细胞经成熟培养和体外受精后,卵裂率为41.8%,但仅27.5%停留在8～16-细胞期,最高发育阶段为16-细胞期;有3层以上卵丘细胞紧密包围的卵丘-卵母细胞复合体(Cumulus-OocyteComplex,COC)经成熟培养和体外受精后,卵裂率(64.5%VS54.7%,p<0.01)和囊胚发育率(34.0%VS18.2%,p<0.01)显著高于卵丘细胞包被不全的卵母细胞。在培养液中添加颗粒细胞虽然没有促进牛受精卵的卵裂率,但提高了囊胚率及其细胞数(p<0.01).

In Vitro Production of Cattle Embryos Derived from Abattoir Ovaries

This experiment was carried out to study effects of collect methods, cumulus cells and granulose cells on developmental competence of bovine oocytes derived from abattoir ovaries following maturation,fertilization and culture in vitro(IVMFC). The result showed that more oocytes could be got in dissected than aspirated method(9.1/ovary vs 6.5/ovary). Oocytes without cumuluc cells(naked oocytes) from the follicles could cleavage following IVMFC. The cleavage rate was 41.8%, but the highest stage was at 16-cell. Both cleavage and blastocyst rates rates of oocytes surrounded by more than 3-layer cumulus cells were significantly higher than oocytes with part cumulus cells(64.5%VS 54.7%; 34.0%VS 18.2%, p<0.01). Although granulose cells had no significantly effect (p>0.05) on the cleavage of bovine embryos, they enhanced the blastocyst rate and cell number (p<0.01).