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转Bar基因紫花苜蓿杂交组合的筛选与评价
引用本文:周仂,王英哲,徐博,谭晶,徐安凯.转Bar基因紫花苜蓿杂交组合的筛选与评价[J].草地学报,2019,27(5):1163-1171.
作者姓名:周仂  王英哲  徐博  谭晶  徐安凯
作者单位:1. 吉林农业大学动物科学技术学院, 吉林 长春 130118;2. 吉林省农业科学院, 吉林 长春 130118
基金项目:优秀青年科学基金;吉林省农业科学院结余经费(统筹部分)资助项目
摘    要:为探究抗除草剂转基因紫花苜蓿(Medicago sativa)的高效杂交种选育技术。本试验以‘公农1号’作为父母本配置了1个对照组合,以转Bar基因的抗草铵膦除草剂转基因紫花苜蓿作为父本,以雄性不育系MS-GN-1A作为母本,配置了11个杂交组合。对后代植株的抗性进行Bar试纸条、PCR检测,对产量和品质性状进行评价和筛选,结果表明,试验中有7个杂交组合的后代表现优异。此试验以紫花苜蓿雄性不育系作为母本配置杂交种,使抗除草剂转基因紫花苜蓿的目标性状高效且稳定遗传,同时在此试验基础上筛选出了优良杂交后代,为良种繁育提供了一定的理论基础。

关 键 词:转基因紫花苜蓿  雄性不育  抗草铵膦  
收稿时间:2018-05-28

Screening and Evaluation of Hybrid Combinations of the Bar Transgenic Alfalfa
ZHOU Le,WANG Ying-zhe,XU Bo,Tan Jing,XU An-kai.Screening and Evaluation of Hybrid Combinations of the Bar Transgenic Alfalfa[J].Acta Agrestia Sinica,2019,27(5):1163-1171.
Authors:ZHOU Le  WANG Ying-zhe  XU Bo  Tan Jing  XU An-kai
Institution:1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, China;2. Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin Province 130118, China
Abstract:To explore and validate the efficient hybrid breeding technology of herbicide-tolerant transgenic alfalfa,one control group was conducted using the alfalfa ‘GN-1’ as the male and female parents;11 hybrid combinations were conducted using 11 glyphosate-resistant herbicide transgenic alfalfa as the male parent and the male sterile line MS-GN-1A as the female parent. The resistance of progeny plants was tested by test paper and PCR,and the yield and quality traits were evaluated and screened. We found that the progenies of 7 hybrid combinations showed excellent performance. Our research used male sterile lines as hybrid seeds to make the target traits of herbicide-tolerant transgenic alfalfa inherit efficiently and stability. Additionally,we have screened out excellent hybrid offspring,which provides a certain theoretical basis for seed breeding.
Keywords:Transgenic alfalfa  Male sterility  Glufosinate resistant  
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