鸡传染性喉气管炎人工感染试验

为建立人工模拟自然感染途径诱发鸡传染性喉气管炎病理模型,本试验采用80日龄京粉鸡随机分成健康对照组和试验组，试验组又设10-4、10-5、10-6 3个不同稀释度的剂量组，每组20只，将鸡传染性喉气管炎病毒经口腔滴注到鸡喉气管内进行攻毒，观察攻毒结果并统计发病和死亡情况。结果表明，10-4攻毒剂量组发病时间为1～8 d，发病率100％,死亡率10％；10-5攻毒剂量组发病时间为2～8 d，发病率100％,死亡率５％；10-6攻毒剂量组发病时间为4～9 d，发病率90％,死亡率0％。各组鸡均表现张口呼吸，咳出带血黏液的临床症状，剖检后均可见气管粘膜肿胀、出血，其中10-5组比较典型。10-5组经喉气管内滴注可以引起鸡100％发病，潜伏期、临床症状和病理变化典型,并且与自然感染基本一致，可用作鸡传染性喉气管炎病理模型。

The Artificial Infection of Infectious Laryngotracheitis in Chickens

In order to establish pathological model of chickens' infectious laryngotracheitis by artificial infection with simulating natural methods.80 days old Jingfen chickens were randomly divided into 1 blank control group and 3 experimental groups with 10-4,10-5,10-6 dosages,20 chickens per group in this paper.The chickens' infectious laryngotracheitis virus(ILTV) was droped into chickens' larynx and trachea through mouth.To be observed the clinical results and statistic the incidence of the disease and the mortality.The results indicate that the 10-4group's delitescence was 1 to 8 days,incidence of the disease was 100%,death rate was 10%;the 10-5group's delitescence was 2 to 8 days,incidence of the disease was 100%,death rate was 5%;the 10-6group's delitescence was 4 to 9 days,incidence of the disease was 90%,death rate was 0%.Per experimental group all can show the same clinical symptoms and pathologic symptom.But the 10-5group is more typical and accord ing with natural infection.So the 10-5group can be used as the artificial pathologic model.