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柑橘青、绿霉病高效拮抗菌34-9的筛选及其特性研究
引用本文:龙超安,邓伯勋,何秀娟. 柑橘青、绿霉病高效拮抗菌34-9的筛选及其特性研究[J]. 中国农业科学, 2005, 38(12): 2434-2439
作者姓名:龙超安  邓伯勋  何秀娟
作者单位:华中农业大学园艺与林学学院/园艺植物生物学教育部重点实验室/国家柑橘育种中心,武汉,430070
基金项目:国家自然科学基金项目(NNSFC-30371018)和湖北省科技计划项目(4006-036030)资助,致谢:承华中农业大学生命科学技术学院梁运祥教授、何绍江教授在菌株鉴定方面的指导.特此感谢.
摘    要: 从柑橘园根际土壤中分离到1株对柑橘青、绿霉病菌有强烈拮抗作用的酵母菌株(34-9),在活体及离体试验中其抑制病菌的作用均较强,在柑橘果实表面接种6 d未发病。其上清液和灭菌液对柑橘青、绿霉病菌没有抑制作用,初步断定该菌株不产生抗生素。经形态特征和生理生化检测,确定其为柠檬形克勒克酵母菌(Kloeckera apiculata)。扫描电镜观察表明,酵母菌株(34-9)对柑橘青、绿霉病菌直接作用,可观察到酵母菌株(34-9)紧紧包围柑橘青、绿霉病菌菌丝或附着吸附在柑橘青、绿霉病菌菌丝上,最终使柑橘青、绿霉病菌菌丝浓缩,菌丝畸形。

关 键 词:柑橘青霉病  柑橘绿霉病  拮抗菌  柠檬形可勒可酵母
收稿时间:2005-01-31
修稿时间:2005-01-31

Characteristics and Identification of an Antagonistic Yeast 34-9 Against Blue and Green Mold of Citrus
LONG Chao-an,DENG Bo-xun,HE Xiu-juan. Characteristics and Identification of an Antagonistic Yeast 34-9 Against Blue and Green Mold of Citrus[J]. Scientia Agricultura Sinica, 2005, 38(12): 2434-2439
Authors:LONG Chao-an  DENG Bo-xun  HE Xiu-juan
Affiliation:Key Laboratory of Horticultural Plant Biology, Ministry of Education/National Center of Citrus Breeding/ College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070
Abstract:A yeast strain was tested for its antagonistic activity against blue mold (Penicillium italicum) and green mold (Penicillium digitatum) of citrus fruit in bean sprout juice agar (BSJDA) medium. The strain (34-9) was identified as Kloeckera apiculata and showed the highest antagonistic activity against citrus blue mold and green mold on pair-culture against P. italicum and P. digitatum of citrus and on wounded citrus fruit. There was no antagonistic activity of fermentated filtrate and after sterilization. Observation by scanning electron microscopy revealed attachment of the yeast cells to the pathogen hyphae, indicating that the high antagonistic activity of the yeast may be contributed by the competition for space and nutrients and /or involvement of direct antagonist of the yeast on the fungus.
Keywords:Penicillium italicum   Penicillium digitatum   Antagonistic microorganism   Kloechera apiculata
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