卵丘细胞对卵母细胞成熟、受精和胚胎发育的影响试验

将从猪卵巢上获取的卵母细胞根据卵丘细胞层数的多少分为两类,并将这两类卵母细胞进行成熟培养、体外受精和孤雌激活,以验证两类卵母细胞的发育潜能.结果显示,将两类卵母细胞分别进行成熟培养时,优级卵的成熟率高于次级卵（67.42±177;1.52％vs 48.33±177;2.85％）,且差异极显著（P＜0.01）;将成熟的卵母细胞进行孤雌激活后,优级卵的囊胚率高于次级卵（58.00±177;2.88％ vs 41.00±177;6.40％）,且差异极显著（P＜0.01）,但囊胚总细胞教两者无显著差异（P＞0.05）;将成熟的卵母细胞进行体外受精后,优级卵的囊胚率、囊胚总细胞数均高于次级卵（18.00土5.70％ vs 4.25±177;2.31％,41.7±177;3.33 vs 36.2土2.10）,且差异极显著（P＜0.01）;进行受精卵孵育时,未去除卵丘细胞的胚胎卵裂率、囊胚率、囊胚总细胞数均高于去除卵丘细胞的（88.14土7.48％ vs 58.69±177;2.89％,51.2±177;7.33％ vs 11.92±177;2.29％,41.7±177;3.33 vs 36.8±177;3.15）,且差异极显著（P＜0.01）.以上结果说明：卵丘细胞层数较多的卵母细胞成熟率高,且其孤雌和体外受精胚胎的发育潜能也好;卵丘细胞存在时,有助于卵母细胞的受精及以后的胚胎发育.

Effects of Cumulus Cells on in Vitro Maturation,Fertilization and Embryo Development of Procine Oocytes

In this study, porcine oocytes collected from overies were divided into two groups according to the thickness of their cumulus cell layer. Group 1 of oocytes have 3 or more than 3 layers of cumulus cells, Group 2 of oocytes have 1-2 layers. These two groups ofoocytes were used for maturation culture, in vitro fertilization 0VF） and partheno-genetical activation （PA） tests to compare their developmental potential. The results showed that oocytes of Group 1 had significantly higher （P〈0.01） maturation rate than that of Group 2 （67.42± 1.52%vs 48.33± 2.85%）, and PA embryos generated from Group 1 oocytes had significantly higher（P〈0.01） blastocyst rate than that of PA embryos produced from Group 2 （58.00± 2.88% vs 41.00± 6.40%） although the blastocyst total cell number was similar （P〉 0.05） between the two groups The blastocyst rate （18.00± 5.70% vs 4.25± 2.31%） and the blastocyst total cell number （41.7± 3.33 vs 36.2± 2.10） of IVF embryos generated from Group I oocytes were significantly higher （P〈0.01 ） than that of Group 2. Moreover, IVF embryos generated from cumulus cells-enclosed oocytes have a higher （P〈0.01） cleavage rate （88.14± 7.48% vs 58.69± 2.89%）, a higher （P〈0.01 ） blastocyst rate （51.2± 7.33% vs 11.92± 2.29%） and higher （P〈0. 01） blastocyst total cell number （41.7±3.33 vs36.8±3.15）, comparedtoIVF embryos produced from cumulus-denuded oocytes. These results suggest that oocytes having thicker layer of cumulus cells bear higher maturation rate and higher embryo developmental ability after IVF and PA, and the presence of cumulus cells during oocyte fertilization was helpful to promote the developmental potential of IVF embryos.