| 马巴贝斯虫18S rRNA基因吉林省分离株的序列分析 |
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| 引用本文： | 张守发,于龙政,熊焕章,张希伟. 马巴贝斯虫18S rRNA基因吉林省分离株的序列分析[J]. 延边大学农学学报, 2006, 28(3): 211-215 |
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| 作者姓名： | 张守发 于龙政 熊焕章 张希伟 |
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| 作者单位： | 1. 延边大学农学院,吉林,龙井,133400
2. 汪清县牧业管理局,吉林,汪清,133200 |
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| 摘 要： | 为分析马巴贝斯虫吉林省分离株的18S rRNA基因序列，根据马巴贝斯虫18S rRNA基因序列设计1对引物，对吉林省的马匹血液基因组DNA进行PCR扩增，PCR产物进行克隆、测序，扩增出大小为435bp的马巴贝斯虫18S rRNA基因片段．序列分析显示，马巴贝斯虫吉林省分离株与南非株同源性最高，为98．4％．
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| 关 键 词： | 马巴贝斯虫 18S rRNA基因 序列分析 |
| 文章编号： | 1004-7999(2006)03-0211-05 |
| 修稿时间： | 2006-04-21 |
Sequence analysis of isolated 18S rRNA gene of Babesia equi in Jilin Province |
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| ZHANG Shou-fa,YU Long-zheng,XIONG Huan-zhang,ZHANG Xi-wei. Sequence analysis of isolated 18S rRNA gene of Babesia equi in Jilin Province[J]. Journal of Agricultural Science Yanbian University, 2006, 28(3): 211-215 |
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| Authors: | ZHANG Shou-fa YU Long-zheng XIONG Huan-zhang ZHANG Xi-wei |
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| Abstract: | To analyse the 18S rRNA nucleotide sequence of Babesia equi in Jilin Province,a pair of primers were designed according to the 18S rRNA gene sequences.The genomic DNA were extracted from horse blood and amplified by PCR.Its purified productions were cloned,(sequenced) and analysed.The results showed that the fragment size was 435 bp,and the homology of nucleotide sequence between Jilin isolated strain and South Africa strain was the highest,which was(98.4%.) |
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| Keywords: | PCR |
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