大麦种质资源抗叶斑病评价

为明确不同大麦种质对叶斑病的抗性差异，筛选抗性种质材料和挖掘相关的遗传位点，在三叶期对200份大麦种质接种叶斑病强致病株Z14484，培养9 d后统计各材料发病情况，明确其抗病等级，进行抗性鉴定与评价。结合Illumina 9K SNP芯片，通过Structure和PLINK软件进行群体和显著位点分析，鉴定与性状显著关联的SNP位点。结果表明，供试大麦材料抗性差异显著，在ΔK=3水平上群体遗传结构可以分为3个亚群，分别包含100、67、33份大麦材料；全基因组关联分析（genome-wide association study，GWAS）共检测到12个与大麦叶斑病抗性相关的位点，其中位于3H染色体上的11_11436和SCRI_RS_201075与大麦叶斑病抗性关联最大。研究结果为大麦叶斑病抗病基因定位与抗病育种提供参考依据。

Evaluation of Resistance to Spot Blotch in Core Germplasm Resources

In order to clarify the resistance differences of different barley germplasms to leaf spot， the resistant germplasm materials were screened and related genetic loci were mined. In this study， 200 barley germplasms were inoculated with Z14484， a highly pathogenic strain of barley leaf spot， at three-leaf stage. After cultured for 9 d， the incidence of each material was counted， the disease resistance grade was clarified， and resistance identification and evaluation were carried out. Combined with Illumina 9K SNP chip， the population and significant loci were analyzed by Structure software and PLINK software to identify SNP loci significantly associated with traits. The results showed that there were significant differences in the resistance among the tested barley materials， and the population genetic structure could be divided into 3 subgroups at ΔK=3 level， including 100， 67 and 33 barley materials， respectively. 11_11436 and SCRI_RS_201075 located on chromosome 3H were most associated with resistance to barley leaf spot by genome-wide association study. This study provided a reference for barley leaf spot disease resistance gene mapping and disease resistance breeding.