不同施氮量下黑果枸杞对干旱胁迫的响应

为明确干旱区栽培黑果枸杞(Lycium ruthenicum Murr.)抗旱生理对施氮(N)的响应,以尿素(含氮量46％)为氮源,于2018-2019年,设置0(CK)、50(N1)、100(N2)、150(N3)和200 g·株-1(N4)共5个施氮量梯度,在干旱胁迫0(土壤含水量30.8％和29.3％)、15(土...

Response of Drought Stress of Lyciumruthenicum Murr. Under Different Nitrogen Applications

To evaluate the physiologic response of drought resistance under different nitrogen （N） applications of Lyciumruthenicum Murr. in arid land， this study used urea （46%） as the N source for 2 years （2018， 2019）， and set 5 N applications including 0 （CK）， 50 （N1）， 100 （N2）， 150 （N3） and 200 g·plant^-1 （N4）， and 3 different degree of drought stresses including 0 （30.8% and 29.3%， soil moiture）， 15 （21.7% and 21.1%， soil moiture） and 30 d （11.2% and 9.7%， soil moiture）. The activities of superoxide dismutase（SOD）， peroxidase （POD） and catalase （CAT） in leaf of Lyciumruthenicum Murr. were measured， and the contents of malondialdehyde （MDA）， proline （Pro） and soluble sugar （SS） were determined. In addition， the drought resistance of Lyciumruthenicum Murr. was comprehensively evaluated by membership function method. The results show that appropriate N application could increase the activities （SOD， POD and CAT） and the contents of Pro and SS content in the leaf of Lyciumruthenicum Murr.， and inhibite the synthesis of MDA. In general， the activities of SOD， POD， CAT and SS content were higher under N3 and N4 treatments， while the contents of Pro were higher and the MDA contents were lower under N2 and N3 treatments. According to the comprehensive evaluation of drought resistance， it showed that the appropriate amount of N application could enhance the drought resistance of Lyciumruthenicum Murr.， but excessive N application should reduce drought resistance. The drought resistant ability were stronger under N2 and N3 treatments after stress 0 and 15 d， and was strongest under N2 treatment after stress 30 d. Therefore， the drought resistance of Lyciumruthenicum Murr. could be improved by applying appropriate amount N in the arid land of Northwest， which provided theoretical basis and technical support for the cultivation pattern of Lyciumruthenicum in arid area.