| 水稻籽粒镉积累KASP分子标记LCd-38的开发与利用 |
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| 引用本文： | 徐君,李婷,胡敏骏,蒋玉根,闫慧莉,许文秀,虞轶俊,何振艳. 水稻籽粒镉积累KASP分子标记LCd-38的开发与利用[J]. 中国农业科技导报, 2022, 24(3): 40-47. DOI: 10.13304/j.nykjdb.2021.1037 |
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| 作者姓名： | 徐君 李婷 胡敏骏 蒋玉根 闫慧莉 许文秀 虞轶俊 何振艳 |
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| 作者单位： | 1.杭州市富阳区农业技术推广中心，杭州 311400;2.中国科学院植物研究所，北方资源植物重点实验室，北京 100093;3.中国科学院大学，北京 100049;4.浙江省耕地质量与肥料管理总站，杭州 310000 |
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| 基金项目： | 国家自然科学基金青年科学基金项目(32000196);科技基础性工作专项(2015FY111300);中国科学院任务/战略性先导科技专项A类(XDA24010404);国家重点研发计划项目(2017YFD0800900) |
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| 摘 要： | 水稻(Oryza sativaL.)是我国主粮作物之一,稻米镉污染对我国粮食安全造成一定威胁.镉低积累水稻品种的选育可有效降低水稻籽粒镉污染风险,高效准确的镉积累相关分子标记在镉低积累水稻品种选育中具有至关重要的作用.以水稻微核心种质为材料,基于籽粒镉积累关联基因的功能性SNP位点开发了KASP分子标记LCd-38.选...
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| 关 键 词： | 水稻 籽粒 镉 KASP分子标记 育种 |
| 收稿时间： | 2021-11-04 |
Development and Utilization of KASP Marker LCd-38 for Cadmium Accumulation in Rice Grain |
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| Jun XU,Ting LI,Minjun HU,Yugen JIANG,Huili YAN,Wenxiu XU,Yijun YU,Zhenyan HE. Development and Utilization of KASP Marker LCd-38 for Cadmium Accumulation in Rice Grain[J]. Journal of Agricultural Science and Technology, 2022, 24(3): 40-47. DOI: 10.13304/j.nykjdb.2021.1037 |
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| Authors: | Jun XU Ting LI Minjun HU Yugen JIANG Huili YAN Wenxiu XU Yijun YU Zhenyan HE |
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| Affiliation: | 1.Agricultural Technology Extention Center in Fuyang Distrcit of Hangzhou, Hangzhou 311104, China;2.Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;3.University of Chinese Academy of Sciences, Beijing 100049, China;4.Zhejiang Station for Management of Arable Land Quality and Fertilizer, Hangzhou 310000, China |
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| Abstract: | Rice （Oryza sativa L.） is one of staple food in China， and the cadmium pollution of rice threatens food security. Screening and breeding of rice varieties with low-cadmium accumulation can effectively reduce the risk， and accurate molecular markers play vital roles in it. In this study， taking the rice mini-core collection as the material， a KASP molecular marker LCd-38 was developed based on the functional SNP site in the gene related to grain cadmium accumulation. The cadmium-polluted soil safe to use was chosen as the experimental area， and LCd-38 was used to genotype and screen rice varieties with low cadmium-accumulation in grain. The developed marker LCd-38 could effectively divide different rice varieties into high-cadmium accumulation genotypes （CC） and low-cadmium accumulation genotypes （TT）. 5 low-cadmium accumulation rice varieties and 5 high-cadmium accumulation rice varieties were quickly identified in the experimental area using leaves at seedling stage， which were consistent with the results of measured grain cadmium concentration at maturity stage. These results indicated that the molecular marker LCd-38 could efficiently and accurately predict the cadmium accumulation characteristics of different rice varieties and be used for early screening and molecular marker-assisted selection breeding of low-cadmium accumulation rice varieties. |
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| Keywords: | Oryza sativa L. grain cadmium KASP molecular marker breeding |
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