巨菌草对七彩山鸡生长、屠宰性能及H-FABP和A-FABP基因表达的影响

本试验通过在基础饲粮中添加巨菌草,探究其对七彩山鸡生长指标、屠宰性能以及心脏型脂肪酸结合蛋白(H-FABP)和脂肪型脂肪酸结合蛋白(A-FABP)基因的影响。试验选取同批次、健康的七彩山鸡150只（4周龄）,随机分为4个试验组和1个对照组,每组10只,3个重复,试验组巨菌草的添加量分别为4%、8%、12%、16%,对照组喂养基础饲粮,饲养周期为16周。结果发现,与对照组相比,添加8%的巨菌草组的平均体重差异显著提高(P<0.05);平均日采食量有所增加,但差异不显著(P>0.05),但各试验组的平均日增重相比较于对照组均显著提高(P<0.05),其中以8%的巨菌草组提高效果最为显著;添加量为4%、8%、12%的巨菌草组料重比有所下降;与对照组相比,8%巨菌草组的屠宰率、半净膛率和全净膛率显著升高(P<0.05);巨菌草组七彩山鸡胸肌率有所升高,但与对照组相比差异不显著(P>0.05);添加量为8%的巨菌草组的腿肌率较对照组显著提高(P<0.05)。经实时荧光定量PCR测定H-FABP和A-FABP基因在添加巨菌草饲粮之后均有差异表达,其中以8%巨菌草组显著提高。因此,饲粮中添加8%的巨菌草可显著改善七彩山鸡的生长指标、屠宰性能和脂肪相关基因的表达。

Effects of Pennisetum giganteum on Growth,Slaughter Performance and Gene Expression of H-FABP and A-FABP in Phasianus colchicus

The purpose of this experiment is to investigate the effect of adding Pennisetum giganteum in diet on growth indexes, slaughter performance, and fat-related genes of heart-type fatty acid-binding protein (H-FABP) and adipocyte fatty acid-binding protein (A-FABP) gene in Phasianus colchicus. The experiment selected 150 healthy P. colchicus (4 weeks old) in the same batch and randomly divided them into 4 experimental groups and 1 control group, 10 in each group, 3 replicates. The addition amount for the experimental groups was 4%, 8%, 12%, and 16%, respectively. The control group was fed with basal diet, and the feeding period was 16 weeks. Compared with that of the control group, the difference of the average body weight of the 8% P. giganteum adding group was significantly increased (P<0.05); the average daily feed intake increased, but the difference was not significant (P>0.05); the average daily weight increase of each experiment group was significantly greater than that of the control group (P<0.05), among which the 8% P. giganteum adding group had the most significant improvement effect. The 4%, 8%, and 12% P. giganteum adding groups had a decrease in the ratio of feed to weight; and compared with those of the control group, the slaughter rate, half evisceration rate and complete evisceration rate of the 8% P. giganteum adding group significantly increased (P<0.05). The breast muscle rate of P. colchicus in the P. giganteum adding groups increased, but the difference was not significant compared with that of the control group (P>0.05); the leg muscle rate in the 8% P. giganteum adding group was significantly higher than that in the control group (P<0.05). The differential expression of H-FABP and A-FABP genes were determined by real-time fluorescence quantitative PCR after the addition of P. giganteum diet, and were significantly increased in the 8% P. giganteum adding group. Therefore, adding 8% of P. giganteum to the diet can significantly improve the growth index, slaughter performance and the expression of fat-related genes in P. colchicus.