甜菜热激蛋白基因BvHSP18.2的克隆和生物信息学分析

旨在通过基因克隆及生物信息学分析，预测甜菜小分子热激蛋白BvHSP18.2 (LOC104903994)的功能。以甜菜‘780016B/12 优’为试验材料克隆 BvHSP18.2，获得 BvHSP18.2 基因全长；通过 ProtParam tool、SWISS-MODEL、MEGA11 等对 BvHSP18.2 的理化性质、蛋白结构、多序列比对等信息进行分析。RT-PCR 扩增得到的 BvHSP18.2 基因全长为 962 bp，编码 158 个氨基酸，分子式为 C₉₂₈H₁₄₆₆N₂₆₆O₂₈₄S₆，属亲水性不稳定的小分子热激蛋白家族；该基因编码蛋白含13个磷酸化位点，α螺旋和无规则卷曲等主要构件。进化关系发现BvHSP18.2与藜麦、菠菜的s HSP同源性相似。同时，BvHSP18.2基因启动子区域具有参与防御和应激反应等顺式作用元件，推测其在甜菜生长发育和胁迫应答中发挥重要作用。本结果为进一步开展该基因的功能和遗传调控机制研究奠定了基础。

Sugar Beet Heat-shock Protein Gene BvHSP18.2: Cloning and Bioinformatics Analysis

The aim of this paper is to predict the function of sugar beet small heat-shock protein BvHSP18.2 (LOC104903994) by gene cloning and bioinformatics analysis. The full length of BvHSP18.2 gene was obtained by cloning BvHSP18.2 from the test material of sugar beet ‘780016B/12 superior’. The physicochemical properties, protein structure and multiple sequence alignment of BvHSP18.2 were analyzed by ProtParam tool, SWISS-MODEL, MEGA11, etc. The full length of BvHSP18.2 gene amplified by RT-PCR was 962 bp, encoding 158 amino acids, with the molecular formula of C₉₂₈H₁₄₆₆N₂₆₆O₂₈₄S₆, which was a hydrophilic unstable protein and belonged to the family of small heat-shock protein family. The protein encoded by this gene contained 13 phosphorylation sites, the alpha helix, irregular curl and other main components. The phylogenetic relationship showed that BvHSP18.2 was similar to the sHSP homologs of quinoa and spinach. Meanwhile, the promoter region of BvHSP18.2 gene had cis-acting elements involved in defense and stress response, and was presumed to play an important role in sugar beet growth and development and stress response. This paper can lay a foundation for further studies on the function and genetic regulation mechanism of this gene.