响应面法优化低聚木糖诱导大豆抗毒素合成条件

为研究大豆抗毒素（glyceollins，GLYs）合成的诱导条件，以低聚木糖（xylooligosaccharides，XOS）诱导黑豆合成的GLYs含量为考察目标，以XOS诱导质量浓度、诱导时间、培养温度为考察单因素，在此基础上采用中心组合设计-响应面法优化GLYs合成量的诱导条件。结果显示，建立的模型适合度显著（F=13.780），GLYs实际合成量与预测值拟合良好（R²=0.925 4）。优化后的最佳诱导条件为XOS诱导质量浓度4.0 g·100 mL^-1，培养温度24 ℃，诱导时间4 d。在此条件下，GLYs合成量为1.376 5 mg·g^-1 DW，与预测值（1.411 8 mg·g^-1 DW）相比，相对误差较小。培养温度对GLYs合成量影响极显著（P<0.05）；XOS诱导质量浓度和诱导时间对GLYs合成量影响不显著；三因素交互作用对GLYs合成量影响均不显著。由此表明，该方法合理可行，为大豆抗毒素制备及功能产品开发提供了理论依据。

Optimization of Glyceollins Synthesis Condition Induced by Xylooligosaccharides Based on Response Surface Methodology

To study condition for inducible synthesis of glyceollins （GLYs）， the content of GLYs synthesized by xylooligosaccharides（XOS） in black soybean was used as an index. And the induction time， XOS mass concentration and temperature were optimized as independent variables. Based on the single factor experiment， central composite design-response surface methodology was employed to optimize inducible condition for GLYs synthesis. The results indicated that the established model fitted significantly well （F=13.780）， and produced GLYs content was matched well with predicted value （R²=0.925 4）. The optimal conditions subjected to optimization were XOS mass concentration 4.0 g·100 mL^-1， temperature 24 ℃， induction time 4 d. Under this condition， the content of accumulated GLYs reached 1.376 5 mg·g^-1 DW. Effect of temperature factor on producing GLYs content （P<0.05） was significant， but XOS mass concentration， induction time and interactions among factors were not significant on GLYs content. Hence， the present method was reasonable and feasible， which provided theoretical basis for glyceollin preparation and functional products development.