| 奶牛隐孢子虫的检测技术 |
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| 引用本文： | 黄燕,徐梅倩,何国声,曹杰,朱顺海.奶牛隐孢子虫的检测技术[J].中国兽医寄生虫病,2006,14(1):32-38. |
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| 作者姓名： | 黄燕 徐梅倩 何国声 曹杰 朱顺海 |
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| 作者单位： | 中国农业科学院上海家畜寄生虫病研究所,上海,200232 |
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| 基金项目： | 科技部"攀登计划";上海市科技兴农科技攻关项目 |
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| 摘 要： | 隐孢子虫病是一种重要的人畜共患原虫病。在家畜中以对反刍动物—奶牛的危害最大。当前，奶牛隐孢子虫主要还是以粪便检测为主，如改良抗酸染色法、金胺一酚改良抗酸复染法等。现在还有应用日趋广泛的免疫学检测方法，如ELISA、IFA、McAb等；分子生物学检测方法。如常规PCR、巢式PCR等。本文对上述各种检测方法作一综述，以供广大寄生虫病学和兽医工作者参考。
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| 关 键 词： | 隐孢子虫病 检测 粪便 |
| 文章编号： | 1005-0868（2006）01-0032-07 |
| 收稿时间： | 2005-06-28 |
| 修稿时间： | 2005年6月28日 |
DETECTIVE TECHNOLOGIES OF CRYPTOSPORIDIUM IN DAIRY CATTLE |
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| HUANG Yan,XU Mei-qian,HE Guo-sheng,CAO Jie,ZHU Shun-hai.DETECTIVE TECHNOLOGIES OF CRYPTOSPORIDIUM IN DAIRY CATTLE[J].Chinese Journal of Veterinary Parasitology,2006,14(1):32-38. |
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| Authors: | HUANG Yan XU Mei-qian HE Guo-sheng CAO Jie ZHU Shun-hai |
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| Institution: | Shanghai Institute of Domestic Animal Parasitology, CAAS , Shanghai 200232, China |
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| Abstract: | Cryptosporidiosis is a important protozonic zoonosis. In livestock, it harms ruminant-dairy catties badly. Now, detecting Cryptosporidium oocysts in dairy cattle is mainly fccai-testing, including classical methods-Modified acid-fast staining, Auramine-phenol staining, immunological methods-ELISA, IFA, McAb, and molecular biological mothods-PCR, Nested PCR and so on. So this article with sum up different kinds of means for detecting. All of these will be supplied as references for personnel of researching parasites. |
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| Keywords: | Cryptosporidiosis detection fecal-testing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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