仔猪大肠杆菌纤毛抗原反向间接血凝检测方法的建立与应用

为建立大肠杆菌K88、K99、987P纤毛抗原的快速鉴别诊断和定量检测方法,用纯化的大肠杆菌K88、K99、987P纤毛抗原免疫家兔制备阳性血清,其琼扩效价分别达到1∶32、1∶32、1∶128;利用该阳性血清IgG致敏醛化的绵羊红细胞,确定了最适K88、K99、987P阳性血清IgG的质量浓度分别为40、160、80μg/mL。用该方法对同一批次的K88、K99和987P纤毛抗原进行3次检测,K88、K99和987P纤毛样品的RIHA效价均为1∶1 600、1∶1 600和1∶800。结果表明,该检测方法具有较好的特异性和可重复性,可用于大肠杆菌K88、K99、987P纤毛抗原的快速鉴别诊断。

Development and application of a reverse indirect hemoagglutination method for detecting piglet E.coli pili antigen

To develop a rapid differentiation and quantitative method for detecting the pili antigen of E.coli K88,K99 and 987P strain,positive serum of rabbit was prepared with purified E.coli K88,K99 and 987P pili antigen and their AGP titers were 1 ∶ 32,1 ∶ 32 and 1 ∶ 128,respectively.Through sensitizing the Aldehydated sheep erythrocyte with the positive serum IgG,the optimal IgG concentrations in K88,K99 and 987P positive sera were determined at 40 μg/mL,160μg/mL and 80μg/mL,respectively.The same batch of K88,K99 and 987P pili antigens were detected three times using the same method and the RIHA titer of K88,K99 and 987P samples were 1 ∶ 1 600,1 ∶ 1 600 and 1 ∶ 800,respectively.The results showed that the detecting method had good specificity and reproducibility and could be used for differentiation and quantitative detection of pili antigen of E.coli K88,K99 and 987P strain.