Hybridization between diploid (Gossypium arboreum) and tetraploid (Gossypium hirsutum) cotton through ovule culture |
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Authors: | Manjeet S Gill Y P S Bajaj |
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Institution: | (1) Punjab Agricultural University, 141004 Ludhiana, India |
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Abstract: | Summary With in vitro culture of ovules, interspecific hybrids have been obtained in an otherwise incompatible cross between a diploid (Gossypium arboreum) and a tetraploid (G. hirsutum) cultivated cotton. The early abortion of the embryo was prevented by repeated treatment of the flowers, immediately after pollination with a solution of gibberellic acid and naphthalene acetic acid. The ovules excised three days after pollination and cultured in a liquid medium underwent profuse proliferation, whereas on an agar-solidified medium supplemented with casein hydrolysate, indoleacetic acid and kinetin they germinated to form hybrid plants. |
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Keywords: | Gossypium arboreum Gossypium hirsutum cotton ovule culture interspecific hybridization |
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