苹果组培苗形成丛枝菌根的过程

以苹果Ｍ２６组培苗为试验材料，以漏斗球囊霉为接种体，进行温室接种试验，经过不同时间取样、染色、压片，观察丛枝菌根的形成过程及其形态结构变化。结果表明：接种２周后就有菌丝侵入，在皮层组织的细胞内开始形成丛枝结构，４周后有胞间泡囊发生。６周后丛枝较密集，８周后有些丛枝开始消解。在皮层细胞间和细胞内形成很多泡囊。１０周后开始有孢子产生。１２周后孢子数量逐渐增多。丛枝菌根着生率，根内泡囊数和根上菌丝着生率

Arbuscular Mycorrhizae Formed Process on the Tissue-Cultured Plantlets of Apple

The tissue-cultured plantlets of apple M26 were inoculated with Glomus mosseae in a greenhouse experiment. The formation process of the arbuscular mycorrhizae (AM) and their morphological change were sampled at different time and observed under microscope by staining. The results showed that at first. the spores and hyphae outside the root served as inoculum and infected the 0.5~-1.0 cm zone behind the growth point on the root tip. The hyphae of G. mosseae began to infect and form arbuscules in conices of the host in 2 weeks after inoculations. The vesicae appeared between the host cells in 4 weeks. The arbuscles became closer in 6 weeks and started to disintegrated. but a lot of vesicae occurred in conices in & weeks. The spores formed and gradually increased in 10 weeks. The infection rate of AM. the vesicae number and the hyphae infection rate in root increased with the extension of time. AM fungi (AMF) mainly distributed in the upper and middle level of the soil and the infection rate and seventy of AMF reduced with the increase of the depth of the soil.