| 长白山赤杨根瘤内Frankia的基因多样性分析 |
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| 引用本文： | 代玉梅,韩士杰,唐晓萌,张成刚.长白山赤杨根瘤内Frankia的基因多样性分析[J].东北林业大学学报,2003,31(6):6-8. |
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| 作者姓名： | 代玉梅 韩士杰 唐晓萌 张成刚 |
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| 作者单位： | 中国科学院沈阳应用生态研究所,沈阳,110016 |
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| 基金项目： | 国家重点基础研究发展规划资助项目(No .0 0 1CB1 0 89)，中国科学院长白山森林生态系统定位研究站资助项目，中国科学院沈阳应用生态研究所创新项目(C1 1 -AG -SCXZY0 1 0 6 ) |
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| 摘 要： | 采集长白山自然保护区北坡东北赤杨、西伯利亚赤杨、色赤杨根瘤样品21个，对根瘤内Frankia DNA的16S-23SrDNA和nifD-nifK两个基因间隔区段(IGS)进行PCR—RFLP分析，研究其基因多样性。结果表明：与赤杨共生的Frankia菌存在丰富的基因多样性，基因类型与宿主种型关系密切。东北赤杨对Frankia的特异性较高，与西伯利亚赤杨和色赤杨共生的Frankia菌有较近的亲缘关系。上述结果说明Frankia菌与其宿主赤杨间存在着协同进化。
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| 关 键 词： | 长白山 赤杨 根瘤 Frankia 基因多样性 协同进化 |
| 修稿时间： | 2003年9月8日 |
Genetic Diversity of Frankia in Nodule of Alnus in Changbai Mountain Revealed by IGS PCR-RFLP |
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| Dai Yumei,Han Shijie,T ang Xiaomeng,Zhang Chenggang.Genetic Diversity of Frankia in Nodule of Alnus in Changbai Mountain Revealed by IGS PCR-RFLP[J].Journal of Northeast Forestry University,2003,31(6):6-8. |
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| Authors: | Dai Yumei Han Shijie T ang Xiaomeng Zhang Chenggang |
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| Abstract: | nodule samples were collected from Alnus mandshurica, Alnus sibirica, and Alnus tinctoria growing in Changbai Mountain. DNA was extracted directly fr om nodules, and its two target DNA regions that encode16S-23S rDNA intergenic sp acer (IGS) and nifD-nifK IGS were studied by PCR-RFLP. The results indicated that rich genetic diversity existed in Alnus-infective Frankia in Chang bai Mountain, and the genotypes were closely related to Alnus species. Aln us mandshurica showed greater specialization towards Frankia, and Franki a strains that infected Alnus sibirica and Alnus tinctoria had more cl ose relationship. Above results suggest the co-evolution between Frankia and its host plants Alnus. |
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| Keywords: | Alnus Frankia Genetic diversity Co-evoluti on |
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