Evaluation of the protective effect of bovine lactoferrin against lipopolysaccharides in a bovine mammary epithelial cell line |
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Authors: | Chiara Pecorini Davide Sassera Raffaella Rebucci Francesca Saccone Claudio Bandi Antonella Baldi |
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Institution: | 1.Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare,Università degli Studi di Milano,Milano,Italy;2.Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria,Università degli Studi di Milano,Milano,Italy;3.Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare,Università degli Studi di Milano,Milano,Italy |
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Abstract: | Lactoferrin (Lf) is a non-haem iron-binding glycoprotein with a molecular weight of about 80 kDa, synthesized by glandular
epithelial cells and stored in the secondary granules of neutrophils. The physiological significance of Lf is related to non-specific
immune defence against pathogens, immunomodulatory activity, iron homeostasis, antioxidant properties and regulation of cell
growth. Lf is a bioactive component of the mammary secretions and its modulatory and defensive functions do affect the newborn
and the mammary gland as well. In this work a bovine mammary epithelial cell line (BME-UV1) was used as an in vitro model
of the bovine mammary epithelium to examine the protective role of exogenous bovine Lf (bLf) against the cytotoxic damage
induced by bacterial lipopolysaccharides (LPS) and the endogenous bLf mRNA expression after LPS exposure. In the in vitro
model used, exogenous bLf exerts a protective effect against endotoxin cytotoxicity, which could be mediated by the LPS-neutralizing
capability of bLf. In addition, in BME-UV1 cells the response to LPS exposure does not involve bLf mRNA expression, suggesting
that this cell line lack of functional LPS-responsive elements. |
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