Evaluation of bacterial strains isolated from oil-contaminated soil for production of polyhydroxyalkanoic acids (PHA) |
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Authors: | Jyotsana Dalal Meeta Lavania Banwari Lal |
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Affiliation: | a Centre for Bioresources and Biotechnology, TERI University, Plot No. 10, Institutional Area, Vasant Kunj, New Delhi 110 070, India b Microbial Biotechnology Division, TERI, Habitat Place, Lodhi Road, New Delhi 110 003, India |
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Abstract: | Samples of eight geographically distinct soils contaminated with crude oil were screened for polyhydroxyalkanoic acid (PHA) producing bacterial strains. Twenty three bacterial strains were able to accumulate PHA when sodium gluconate or sodium octanoate was used as the sole carbon source. Biochemical tests and 16S rRNA sequencing identified bacteria of the genera Pseudomonas, Acinetobacter, Sphingobacterium, Brochothrix, Caulobacter, Ralstonia, Burkholderia and Yokenella. Three of the bacterial strains have never been reported to produce PHA. The phylogenetic analysis of the PHA synthase (phaC) gene of these bacteria showed a close homology with the phaC gene of different Pseudomonas species.This study indicates that stressed environments like oil-contaminated sites can be potential sources of medium-chain-length PHA producers. |
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Keywords: | Polyhydroxyalkanoic acids Bacteria Oil-contaminated soil |
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