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Potential novel markers to discriminate between active and latent tuberculosis infection in Chinese individuals
Institution:1. Army Tuberculosis Prevention and Control Key Laboratory, Beijng Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute for Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, PR China;2. Department of Pathology, The 309th Hospital of Chinese PLA, Beijing 100091, PR China;3. Department of Respiratory, The 309th Hospital of Chinese PLA, Beijing 100091, PR China;4. Department of Orthopedics, The 309th Hospital of Chinese PLA, Beijing 100091, PR China;1. Research Institute of Life Science, Gyeongsang National University, Jinju 52828, South Korea;2. Division of Life Science, Gyeongsang National University, Jinju 52828, South Korea;1. Department of Pathogenic Organisms, Chongqing Medical University, Chongqing, China;2. Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing, China;3. Department of Respiratory Disease, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China;1. Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark;2. Department of Infectious Diseases and Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, Freiburg, Germany;3. Department of Double Diagnosis, Mental Health Centre Sct. Hans, Roskilde, Denmark;4. Division of Clinical Infectious Diseases, Research Center Borstel, Germany;5. German Center for Infection Research Tuberculosis Unit International Health/Infectious Diseases, University of Lübeck, Germany;6. Department of Internal Medicine, University of Namibia School of Medicine, Windhoek, Namibia;7. Department of Medicine, Karolinska Institute, Stockholm, Sweden;8. Department of Pulmonary and Infectious Diseases, Nordsjaelland Hospital, Hillerød, Denmark;9. Tuberculosis Outpatient Centre Vila Nova de Gaia, Portugal;10. Hospital Centre of Vila Nova de Gaia/Espinho, Portugal;11. Medical School, Porto University, Portugal;12. Institute of Public Health, Porto University, Portugal;13. Pulmonology Diagnostic Center Porto and Vila Nova de Gaia, Portugal;14. Division of Infectious Diseases and Hospital Epidemiology, Cantonal Hospital St.Gallen, Switzerland;15. Division of Pneumology, Department of Internal Medicine, Cantonal Hospital St.Gallen, Switzerland;p. Servei de Microbiologia, Institut d’Investigació en Ciències de la Salut Germans Trias i Pujol, Universitat Autònoma de Barcelona, Ciber Enfermedades Respiratorias, Instituto de Salud Carlos III, Badalona, Spain;1. School of Pathology and Laboratory Medicine, University of Western Australia, Perth, Australia;2. Tuberculosis Control Program, Health Department of Western Australia, Perth, Australia;3. Centre for Asthma, Allergy and Respiratory Research, Lung Institute of Western Australia, Perth, Australia;4. INSERM U955, Institut Mondor de Recherche Biomédicale, Créteil, France;5. Vaccine Research Institute (VRI) and Université Paris-Est Créteil, France;6. Department of Clinical Immunology and PathWest Laboratory Medicine, Royal Perth Hospital, Perth, Australia;1. Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Immunology, Shanghai, 200025, China;2. Shanghai Public Health Clinical Center, Key Laboratory of Medical Molecular Virology of MOE/MOH, Fudan University, 2901 Caolang Rd., Shanghai, 201508, China;3. Department of Microbiology, School of Life Sciences, Fudan University, Shanghai, 200438, China;4. Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, 201200, China
Abstract:Latent tuberculosis infection (LTBI) constitutes the main reservoir for reactivation tuberculosis. The finding of potential biomarkers for differentiating between TB and LTBI is very necessary. In this study, the immunological characteristics and potential diagnostic utility of Rv2029c, Rv2628 and Rv1813c proteins were assessed. These three proteins stimulated PBMCs from ELISPOT-positive LTBI subjects produced higher levels of IFN-γ in comparison with TB patients and ELISPOT-negative healthy subjects (p < 0.05). BCG vaccination and non-TB respiratory disease had little influence on the immunological responses of Rv2029c and Rv2628 proteins (p > 0.05). The LTBI diagnostic performance of Rv2029c was higher than Rv2628 and Rv1813c by ROC evaluation. But Rv2628 had much higher specificity than Rv2029c in active TB patients and uninfected healthy subjects. The IgG level against Rv1813c was higher in the TB group than in LTBI and uninfected healthy subjects (p < 0.05). These results suggest that T cell response to Rv2628 and antibody against Rv1813c might be applicable as biomarkers to distinguish TB from LTBI and uninfected individuals.
Keywords:Latent tuberculosis infection  Mycobacterium tuberculosis  Tuberculosis  Rv2029c  Rv2628  Rv1813c  Diagnosis
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