Development of an assessment key and techniques for field screening of tea (Camellia sinensis L.) cultivars for resistance to blister blight |
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| Institution: | 1. Plant Pathology Division, Tea Research Institute of Sri Lanka, Talawakelle 22100, Sri Lanka;2. Plant Breeding Division, Tea Research Institute of Sri Lanka, Talawakelle 22100, Sri Lanka;3. Department of Agriculture Biology, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya 81100, Sri Lanka;1. LAQV/REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal;2. CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal;1. Key Laboratory of Quantitative Remote Sensing in Agriculture of Ministry of Agriculture, Beijing Research Center for Information Technology in Agriculture, Beijing 100097, China;2. National Engineering Research Center for Information Technology in Agriculture, Beijing 100097, China;3. Beijing Engineering Research Center for Agriculture Internet of Things, Beijing 100097, China;4. Key Laboratory of Agri-informatic, Ministry of Agriculture, Beijing 100097, China;5. National Engineering Research Center for Agro-Ecological Big Data Analysis & Application, Anhui University, Hefei 230601, China;1. Università degli Studi di Milano, Department of Food, Environmental and Nutritional Sciences, Via Celoria 2, 20133 Milano, Italy;2. Plant Pathology Research Institute, Agricultural Research Center, 9 Gamaa St., 12619 Giza, Egypt;1. Department of Botany, Gauhati University, Guwahati 781014, Assam, India;2. Department of Ecology and Environmental Science, Assam University, Silchar 788011, Assam, India;3. Agroprocessing and Natural Products Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Thiruvananthapuram 695019, Kerala, India;1. National Engineering Research Center for Agro-Ecological Big Data Analysis & Application, Anhui University, Hefei 230601, China;2. School of Information Science and Technology, Fudan University, Shanghai 200433, China |
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| Abstract: | Blister blight caused by the biotrophic fungus, Exobasidium vexans Massee, is the most problematic foliar disease of tea in Sri Lanka. A reliable and accurate method is needed for field assessment of severity of the disease for epidemiological studies, formulating disease control strategies and crop improvement programmes. A field assessment key with 0–6 scores was developed for blister blight, considering the lowest (0) and highest (>30%) limits of disease severity observed in the field and different stages of symptom development. The key was validated by six raters, 3 experienced and 3 inexperienced. The field assessment trials made using the key were accurate and precise (R2 > 0.80). The area under the disease progress curve (AUDPC), calculated using the disease severity levels obtained using the assessment key, was used to combine multiple observations of disease progress into a 0–9 susceptible scale. Ascending numbers in the scale represent increasing susceptibility. The new scale was proposed to discriminate blister blight resistance in tea accessions/cultivars in field screening. Screening trials for validation of the susceptible scale, conducted using tea cultivars of known resistance or susceptibility levels and newly developed accessions of tea, at three locations, revealed that the 0–9 scale is simple to apply, offers a fine discrimination of blister blight resistance levels, and allows objective evaluation. |
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| Keywords: | AUDPC Disease assessment Severity index Susceptible scales Varietal screening |
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