A minimally invasive approach to spleen histopathology in dogs: A new method for follow-up studies of spleen changes in the course of Leishmania infantum infection |
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| Institution: | 1. Fundação Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz, Salvador, BA, Brazil;, Brazil;2. Laboratório de Infectologia Veterinária, Hospital de Medicina Veterinária, Universidade Federal da Bahia (UFBA), Salvador, BA, Brazil;3. Departmento de Anatomia, Patologia and Clínicas, Escola de Medicina Veterinária e Zootecnia, UFBA, Salvador, BA, Brazil;4. Faculdade de Ciências Médicas, UNICAMP, Campinas, SP, Brazil;1. Child and Adolescent Heath Program, Faculty of Medical Sciences, State University of Campinas, Campinas, São Paulo, Brazil;2. University of Ferrara, Ferrara, Italy;1. Servicio de Cirugía Ortopédica y Traumatología, Hospital Universitario Infanta Leonor, Madrid, España;2. Unidad de Cirugía de Mano, Hospital Beata María Ana, Madrid, España;3. Departamento de Cirugía, Facultad de Medicina, Universidad Complutense de Madrid, España;4. Centro de Donación de Cuerpos y Salas de Disección, Facultad de Medicina, Universidad Complutense de Madrid, España;5. Conento, Madrid, España;1. Immune Section, Research Institute for Animal Reproduction, Cairo, Egypt;2. Department of Toxicology, Forensic Medicine and Veterinary Regulations, Faculty of Veterinary Medicine, Cairo University, Egypt;3. Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Egypt;1. Department of Obstetrics and Gynecology, Washington University School of Medicine, 4566 Scott Ave., St. Louis, MO, 63110, USA;2. Department of Obstetrics and Gynecology, University of Campinas, Campinas, Brazil;3. Department of Physiology and Biophysics and Center for Cardiovascular Research, University of Illinois, Chicago, Illinois 60612;4. Department of Surgery, Committee on Cellular and Molecular Physiology, University of Chicago, Chicago, Illinois 60637;1. São Leopoldo Mandic Dental School and Research Center, Rua José Rocha Junqueira 13, Bairro Swift, Campinas, CEP: 13045-755, São Paulo, Brazil;2. Institute of Science and Technology of São José dos Campos, UNESP—Universidade Estadual Paulista, Avenida Engenheiro Francisco José Longo, 777, Jardim São Dimas, São José dos Campos, CEP: 12245-000, São Paulo, Brazil;3. Faculty of Medical Science (FCM), University of Campinas (UNICAMP), Rua Tessália Vieira de Camargo, 126 — Cidade Universitária Zeferino Vaz, Campinas, CEP: 13083-887, São Paulo, Brazil |
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| Abstract: | Severe forms of zoonotic visceral leishmaniosis (ZVL) are associated with disruption of the spleen structure. However, the study of spleen histology requires splenectomy or necropsy. In this work, we present a minimally invasive cell-block technique for studying spleen tissue histology in dogs with ZVL. We examined 13 dogs with and seven dogs without Leishmania infantum infection. The dogs with Leishmania infection had a lower frequency of lymphoid follicles (2/13, Fisher’s test, P < 0.02) and a higher density of plasma cells (score 3, Fisher’s test, P < 0.02) than uninfected dogs (5/7 exhibiting lymphoid follicles and a plasma cell score of 1). The dogs with Leishmania infection also presented with granulomas (8/13) and infected macrophages (5/13). These differences in the histological presentations of spleen tissue from infected and uninfected dogs corresponded to changes observed in conventional histology. Hence, the cell-block technique described here may be used in the follow-up care and study of dogs with ZVL and other diseases in both clinical practice and research. |
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| Keywords: | Dog Canine visceral leishmaniasis Spleen Cell block Fine-needle biopsy Minimally invasive histopathology |
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