Immunological evaluation of an rsmD-like rRNA methyltransferase from Wolbachia endosymbiont of Brugia malayi |
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| Affiliation: | 1. Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria, Australia;2. Australian National Wildlife Collection, Commonwealth Scientific and Industrial Research Organisation, Canberra, Australia;3. Pathology Queensland, Townsville Hospital, Queensland, Australia;4. Department of Microbiology and Infectious Diseases, Royal Hobart Hospital, Tasmania, Australia;5. Department of Neurology, The Townsville Hospital, Queensland, Australia;6. Anatomical Pathology, Royal Brisbane & Women''s Hospital, Herston, Queensland, Australia;7. Department of Molecular and Cellular Pathology, School of Medicine, University of Queensland, Queensland 4006, Australia;8. Electron Microscope Unit, Pathology Queensland, Royal Brisbane & Women''s Hospital, Queensland, Australia;9. School of Medical and Applied Sciences, Central Queensland University, Rockhampton, North Rockhampton, Queensland, Australia |
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| Abstract: | Wolbachia is a wonderful anti-filarial target with many of its enzymes and surface proteins (WSPs) representing potential drug targets and vaccine candidates. Here we report on the immunologic response of a drug target, rsmD-like rRNA methyltransferase from Wolbachia endosymbiont of Brugia malayi. The recombinant protein generated both humoral and cell-mediated response in BALB/c mice but compromised its immunity. The humoral response was transient and endured barely for six months in mice with or without B. Malayi challenge. In splenocytes of mice, the key humoral immunity mediating cytokine IL4 was lowered (IL4↓) while IFNγ, the major cytokine mediating cellular immunity was decreased along with upregulation of IL10 cytokine (IFNγ↓, IL10↑). The finding here indicates that the enzyme has low immunogenicity and triggers lowering of cytokine level in BALB/c mice. Interestingly the overall immune profile can be summed up with equivalent response generated by WSP or whole Wolbachia. |
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| Keywords: | rsmD-like rRNA-methyltransferase Immune response BALB/c |
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