非AA型野生稻叶绿体DNA籼粳特性研究

籼粳分化现象广泛存在于亚洲栽培稻（O．sativa）中。大量研究表明，普通野生稻（O．rufipogon）的叶绿体DNA也存在籼粳分化。为进一步探明非AA型野生稻的叶绿体DNA是否存在籼粳分化现象，利用2个长度多态性籼粳分型标记（ORF100和ORF29-TmC^GCA）对12个非AA型野生稻种的叶绿体DNA进行籼粳特性分析。研究发现，非AA型野生稻叶绿体DNA都呈现偏粳趋势。对叶绿体DNA碱基多态性最丰富的2个区域（rps16基因内含子和TmT^UGU-TmL^UAA间区）进行测序比较，在4个位点的籼粳分型标记中，非AA型野生稻有3个位点与粳型标记一致，1个位点与籼型标记一致，但另有多个位点的碱基与栽培稻不同。研究结果表明，非AA型野生稻叶绿体DNA总体偏粳，但与典型粳稻存在一定遗传差异。推测粳型叶绿体可能为稻属原始类型。

Study on Indica-japonica Character of Non-AA Wild Rice Chloroplast DNA

Differentiation of indica-japonica character in O. sativa commonly exists. A great deal of research showed the chloroplast DNA of O. rufipogon also exhibit diversity of indica-japonica character. In order to prove whether the chloroplast DNA of non-AA wild rice shows differentiation of indica-japonica character, this study analyzed the chloroplast DNA of non-AA wild rice using molecular markers for separating indica-japonica traits. The chloroplast DNA of non-AA wild rice was japonica-type using two length diversity markers (ORF100 and ORF29-TrnCGCA). Utilizing four sequence markers exited in intron of rps16 gene and TrnTUGU-TrnLUAA spacer, the result of three markers showed that the chloroplast DNA of non-AA wild rice were japonica-type and the result of one marker showed that they were indica-type. It also showed that differentiation existed between the sequence of tested material and the markers, and some mutation has happened. The result show japonica-type may be the original type of rice chloroplast DNA.