| 草鱼呼肠孤病毒VP6蛋白基因植物表达载体的构建 |
| |
| 引用本文： | 周勇,范玉顶,徐进,李艳秋,肖艺,曾令兵. 草鱼呼肠孤病毒VP6蛋白基因植物表达载体的构建[J]. 淡水渔业, 2009, 39(4) |
| |
| 作者姓名： | 周勇 范玉顶 徐进 李艳秋 肖艺 曾令兵 |
| |
| 作者单位： | 华中农业大学水产学院,武汉,430070;中国水产科学研究院长江水产研究所,湖北荆州,434000;中国水产科学研究院长江水产研究所,湖北荆州,434000;华中农业大学水产学院,武汉,430070 |
| |
| 基金项目： | 农业公益性行业科研专项 |
| |
| 摘 要： | 以据草鱼呼肠孤病毒(GCRV)VP6蛋白的全基因序列(GeneBank AF403394)为模板,采用RT-PCR构建了草鱼呼肠孤病毒VP6蛋白基因植物表达载体的构建。结果显示:实验构建的pCR 2.1-VP6重组质粒含有NcoⅠ和BglⅡ酶切位点;pCR 2.1-VP6重组质粒经PCR扩增和测序显示含有1.3 Kbp的草鱼呼肠孤病毒VP6基因读码框片段。将目的片段VP6酶切、克隆到携带有绿色荧光蛋白(GFP)基因的植物表达载体pCAMBIA1302中,再经酶切、PCR扩增和序列测定显示,pCAMBIA1302-VP6含有1.3 Kbp的草鱼呼肠孤病毒VP6基因片段,说明已插入植物表达载体pCAMBIA1302绿色荧光蛋白基因前,成功构建了融合表达草鱼呼肠孤病毒VP6蛋白和绿色荧光蛋白的植物表达载体pCAMBIA1302-VP6。
|
| 关 键 词： | 草鱼呼肠孤病毒 VP6基因 植物表达载体 构建 |
Construction of plant-based expression vector of grass carp reovirus VP6 gene |
| |
| ZHOU Yong,FAN Yu-ding,XU Jin,LI Yan-qiu,XIAO Yi,ZENG Ling-bing. Construction of plant-based expression vector of grass carp reovirus VP6 gene[J]. Freshwater Fisheries, 2009, 39(4) |
| |
| Authors: | ZHOU Yong FAN Yu-ding XU Jin LI Yan-qiu XIAO Yi ZENG Ling-bing |
| |
| Affiliation: | ZHOU Yong1,2,FAN Yu-ding2,XU Jin2,LI Yan-qiu2,XIAO Yi1,ZENG Ling-bing1,2(1.Fisheries College of Huazhong Agricultural University,Wuhan 430070,2.Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Jingzhou,Hubei 434000) |
| |
| Abstract: | The coding region of grass carp reovirus(GCRV) VP6 gene was amplified by RT-PCR with primers based on the gene sequence in GeneBank(AF403394) from the viral RNA genome extracted in virus infected Ctenopharyngodon idellus Kidney(CIK) cells.1.3 kb PCR product was ligated to pCR 2.1 vector,and after identified with enzyme digestion,PCR detection and sequencing,then the 1.3 kb cording sequence of GCRV VP6 gene was cloned into the plant-based expression vector pCAMBIA1302which had a green fluorescent protein(GFP... |
| |
| Keywords: | grass carp reovirus VP6 gene plant-based expression vector construction |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
