Mapping genes for double podding and other morphological traits in chickpea |
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Authors: | Seungho Cho Jagdish Kumar Jeff L Shultz K Anupama F Tefera Fred J Muehlbauer |
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Institution: | (1) School of Molecular Biosciences and the Grain Legume Genetics and Physiology Research Unit, USDA-ARS, Pullman, Washington State University, WA, 99164-6434, U.S.A;(2) International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Andhra Pradesh, 502 324, India |
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Abstract: | Seed traits are important considerations for improving yield and product quality of chickpea (Cicer arietinum L.). The purpose of this study was to construct an intraspecific genetic linkage map and determine map positions of genes
that confer double podding and seed traits using a population of 76 F10 derived recombinant inbred lines (RILs) from the cross of ‘ICCV-2’ (large seeds and single pods) × ‘JG-62’ (small seeds and
double podded). We used 55 sequence-tagged microsatellite sites (STMS), 20 random amplified polymorphic DNAs (RAPDs), 3inter-simple
sequence repeats (ISSR) and 2 phenotypic markers to develop a genetic map that comprised 14 linkage groups covering297.5 cM.
The gene for double podding (s) was mapped to linkage group 6 and linked to Tr44 and Tr35 at a distance of7.8 cM and 11.5 cM, respectively. The major gene
for pigmentation, C, was mapped to linkage group 8 and was loosely linked to Tr33 at a distance of 13.5 cM. Four QTLs for 100 seed weight (located
on LG4 and LG9), seed number plant-1 (LG4), days to 50% flower (LG3) were identified. This intraspecific map of cultivated chickpea is the first that includes
genes for important morphological traits. Synteny relationships among STMS markers appeared to be conserved on six linkage
groups when our map was compared to the interspecific map presented by Winter et al. (2000).
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Cicer arietinum genome mapping genetic linkage map morphological trait QTL STMS |
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