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鸡Nramp1基因第9外显子的单核苷酸序列多态性分析
引用本文:胡国顺,常国斌,王克华,陈蓉,刘艳,周琼,廖菁,陈国宏. 鸡Nramp1基因第9外显子的单核苷酸序列多态性分析[J]. 中国畜牧兽医, 2010, 37(6): 119-121
作者姓名:胡国顺  常国斌  王克华  陈蓉  刘艳  周琼  廖菁  陈国宏
作者单位:(1.扬州大学动物科学与技术学院, 扬州 225009; 2.中国农业科学院家禽研究所, 扬州 225003)
基金项目:国家"十一五"863重点项目,国家"十一五"科技支撑计划重点项目 
摘    要:以文昌鸡、安卡鸡及藏鸡为试验动物,利用PCR-SSCP方法检测Nramp1基因部分区域的单核苷酸多态性(SNP)。结果发现,该区域存在2个突变,分别为C315G、C357T;共出现4种基因型,分别为GG、GT、TT及AA型,在文昌鸡和藏鸡群体内,T为优势等位基因,而安卡鸡中G为优势等位基因。3个群体该座位均处于哈代-温伯格平衡(PO.05),且3个群体间基因型分布无显著差异(P0.05)。

关 键 词:  Nramp1基因  单核苷酸多态性  

Single Nucleotide Polymorphisms (SNPs) in Exon 9 of Nramp1 Gene of Chickens
HU Guo-shun,CHANG Guo-bin,WANG Ke-hua,CHEN Rong,LIU Yan,ZHOU Qiong,LIAO Jing,CHEN Guo-hong. Single Nucleotide Polymorphisms (SNPs) in Exon 9 of Nramp1 Gene of Chickens[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(6): 119-121
Authors:HU Guo-shun  CHANG Guo-bin  WANG Ke-hua  CHEN Rong  LIU Yan  ZHOU Qiong  LIAO Jing  CHEN Guo-hong
Affiliation:(1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;2.Institute of Poultry Science,Chinese Academy of Agricultural Sciences,Yangzhou 225003,China)
Abstract:Wenchang,Anka and Zang chickens were used for SNPs discovery in exon 9 of Nramp1 gene using PCR-SSCP and sequencing methods. Two SNPs at C315G and C357T,and four genotypes of GG,GT,TT and AA were detected in three chicken populations. Allele T was the dominant allele in Wenchang and Zang chicken populations,while allele G was the dominant allele in Anka chicken population. All the populations were in Hardy-Weinberg equilibrium(P>0.05).There were no significant differences in three chicken populations(P>0.05).
Keywords:chicken  Nramp1 gene  SNP
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