cDNA文库构建策略及其在盐生植物中的应用

构建各种cDNA文库是目前发现新基因的基本策略,同时也是功能基因组学研究的一个主要方法.cDNA文库主要包括cDNA全长文库、cDNA差减文库和cDNA均一化文库等类型,各种文库的原理、构建方法和适用范围都有很大的不同.近年来,通过构建各种cDNA文库、获得大规模高质量EST数据库,并在此基础上分离克隆抗逆相关基因已经成为研究植物耐盐机理的重要途径.旨在对cDNA文库的主要类型及其构建策略,以及cDNA文库在盐生植物新基因筛选中的应用进行综合论述,并对植物耐盐机制的研究提出了展望.

Strategies of cDNA Library Construction and Its Application in Halophytes

cDNA library construction is a basic way of new gene discovery,it is also an important approach of genomics study.At present,the types of cDNA libraries include full-length cDNA library、cDNA subtractive library and normalized cDNA library,there are great differences in the principle,construction methods and their applications.Recently,the great high quality EST data bank has been developed with construction of different cDNA libraries,on the basis of which the resistance genes were cloned and separated.The objective of this paper was to discuss the main types and construction strategies of cDNA and its application to halophytes and salt-tolerant mechanism of halophytes were suggested.