| 酶免疫法测定磺胺喹恶啉残留的研究：磺胺喹恶啉抗体的制备 |
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| 引用本文： | 刘智宏,吴贤福.酶免疫法测定磺胺喹恶啉残留的研究：磺胺喹恶啉抗体的制备[J].中国兽药杂志,1997,31(4):17-18. |
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| 作者姓名： | 刘智宏 吴贤福 |
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| 作者单位： | 中国兽药监察所 |
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| 摘 要： | 磺胺喹恶啉（ＳＱ）常用于鸡球虫病的防治，但它有一定的毒性，因此该化合物在肉类食品中的残留日益受到人们的关注。本试验用戊二醛法将ＳＱ与牛血清白蛋白（ＢＳＡ）联接形成完全抗原，用紫外扫描对其进行鉴定，并测定其ＳＱ与ＢＳＡ的分子比为１２．１∶１。随后将此抗原免疫家兔制备高效价的抗ＳＱ血清，并用ＥＬＩＳＡ法和琼脂扩散法对其进行鉴定。
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| 关 键 词： | 磺胺喹恶啉 酶免疫法 残留 |
Study on Enzyme Immunoassay for Measuring of Sulfaquinoxaline (SQ)residue Preparation of Antibody against SQ |
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| Liu Zhihong,Wu Xianfu,Sun Ye,Wan Renlin National Institute for Control of Veterinary Bioproducts and Pharmaceuticals Beijing.Study on Enzyme Immunoassay for Measuring of Sulfaquinoxaline (SQ)residue Preparation of Antibody against SQ[J].Chinese Journal of Veterinary Drug,1997,31(4):17-18. |
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| Authors: | Liu Zhihong Wu Xianfu Sun Ye Wan Renlin National Institute for Control of Veterinary Bioproducts and Pharmaceuticals Beijing |
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| Institution: | Liu Zhihong,Wu Xianfu,Sun Ye,Wan Renlin National Institute for Control of Veterinary Bioproducts and Pharmaceuticals Beijing 100081 |
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| Abstract: | Sulfaquinoxaline(SQ)is mainly used in control of chiken coccidosis. Because of its toxicity, much attention has been paid to SQ residue in meat products. In this paper, the authors linked SQ to bovine serum albumin by glutaraldehyde to form complete antigen. The complex was determined by UV spectrametric scanning method and the molecular ratio of SQ to BSA was 12.1. I t was used to immunize rabbits. High titer antiserum against hapten SQ havesed, which was confirmed by ELISA and agar precipitating test. |
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| Keywords: | Sulfaquinoxaline (SQ) ELISA |
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