冰核细菌对杏花器官抗寒性的影响

 从田间采取两个仁用杏品种的大蕾期花枝, 在室内进行水培。对花器官人工接种冰核( INA)细菌, 置于模拟自然霜夜的半导体制冷电脑控温和自动记录的人工霜箱内, 研究INA 细菌对花器官抗寒性的影响。结果表明: 杏花器官抗寒性顺序为花瓣> 雄蕊> 雌蕊。未接菌仁用杏花器官能抵抗- 4～ - 6 ℃低温, 而INA 细菌能提高仁用杏花器官相对电导率, 破坏膜保护酶SOD、POD 的活性, 使细胞积累大量自由基, 并增强膜脂过氧化作用, 加剧MDA 含量的积累, 使花器官发生严重褐变乃至死亡。INA 细菌能在- 2～ - 3 ℃诱发细胞结冰, 使组织冰点提高2 ℃左右, 因此, INA 细菌可诱发和加重霜冻害。

The Effect of Ice Nucleation Active Bacteria on Cold Resistance of Apricot Flower Organs

Abstract : The hardiness of apricot flower organs were investigated with two varieties , by comparing the cold resistance. The obvious difference among the apricot flower organs and varieties with or without INA bacteriainoculation were observed. The variety Youyi had higher cold hardiness than Longwangmao. In addition , tests on the cold hardiness difference in various organs showed petal > stamen > pistal . Under stress of low temperatures ,treatment of bacteria can greatly raise relative electric conductivity , permeability of cell membrane , brown change and content of MDA , and cut down SOD , POD activity of apricot organs. The treated flower organs showedsymptoms of serious frost at - 2 to - 3 ℃, and had supercooling points of - 2 ℃about higher than controls. The results demonstrated that INA bacteria are one major factor to incite frost damage to apricot flower organs , so duringflowering phage , frost injury may be relieved through control of INA bacteria.