The <Emphasis Type="Italic">cyv-2</Emphasis> resistance to <Emphasis Type="Italic">Clover yellow vein virus</Emphasis> in pea is controlled by the eukaryotic initiation factor 4E |
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Authors: | Marcelo Andrade Yosuke Abe Kenji S Nakahara Ichiro Uyeda |
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Institution: | (1) Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan |
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Abstract: | The same mutant allele of eukaryotic initiation factor 4E (eIF4E) that confers resistance to Pea seed-borne mosaic virus (sbm-1) and the white lupine strain of Bean yellow mosaic virus (wlv) also confers resistance to Clover yellow vein virus (ClYVV) in pea. The eIF4E genes from several pea lines were isolated and sequenced. Analysis of the eIF4E amino acid sequences from several resistant
lines revealed that some lines, including PI 378159, have the same sequence as reported for sbm-1 and wlv. When eIF4E from a susceptible pea line was expressed from a ClYVV vector after mechanical inoculation of resistant PI 378159, the virus
caused systemic infection, similar to its effects in susceptible line PI 250438. The resistance to ClYVV in line PI 378159
was characterized through a cross with PI 193835, which reportedly carries cyv-2. Mechanical inoculation of the F1 progeny with ClYVV resulted in no infection, indicating that the resistance gene in PI
378159 is identical to cyv-2 in PI 193835. Furthermore, particle bombardment of pea line PI 193835 with infectious cDNA of ClYVV (pClYVV/C3-S65T) resulted
in the same resistance mode as that described for PI 378159. These results demonstrate that the resistance to ClYVV conferred
by cyv-2 is mediated by eIF4E and that cyv-2 is identical to sbm-1 and wlv. |
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Keywords: | Clover yellow vein virus cyv-2 sbm-1 wlv Eukaryotic initiation factor 4E Pea resistance |
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