水稻Wx复等位基因的鉴定及单片段代换系的建立

 利用微卫星标记“484/485”和“484/W2R”结合AccⅠ酶切对278份来自国内外水稻品种（系）进行了Wx座位复等位基因分析，共检测到15种等位基因，其中(CT)12-G、(CT)15-G、(CT)16-G、(CT)17-G、(CT)18-G、(CT)21-G为新发现的等位基因。以含不同Wx等位基因的20个品种（系）为供体亲本，利用回交和微卫星标记辅助选择相结合的方法，建立了72个以Wx等位基因为 (CT)11-G 的“华粳籼74”为受体的Wx复等位基因单片段代换系。这些单片段代换系共包含12种Wx等位基因，其代换片段长度最短为2.2 cM，最长为77.3 cM，平均为17.4 cM。

Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines in Rice

The microsatellite markers 484/485 and 484/W2R were used to identify the multiple alleles at the Wx locus in rice germplasm. Fifteen alleles were identified in 278 accessions by the use of microsatellite class and G-T polymorphism. Among them, (CT)_ 12-G, (CT)_ 15-G, (CT)_ 16-G, (CT)_ 17-G, (CT)_ 18-G and (CT)_ 21-G are new ones. Seventy-two single segment substitution lines (SSSLs) carrying different alleles at the Wx locus were developed by the use of Huajingxian 74 with the (CT)_ 11-G allele as a recipient and 20 varieties containing 12 alleles at the Wx locus as donors. The estimated length of the substituted segments ranged from 2.2 cM to 77.3 cM with an average of 17.4 cM.