Comparison of gene delivery methods for transformation ofRobinia pseudoacacia L. by Ti-plasmid |
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Authors: | Ren Chen Koichiro Gyokusen Akira Saito |
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Institution: | (1) Department of Forestry, Faculty of Agriculture, Kyushu University, 812-8581 Fukuoka, Japan |
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Abstract: | The binary vector plasmid pIG121-Hm carrying the chimeric neomycin phosphotransferase (npt II), β-glucuronidase (gusA) and hygromycin phosphotransferase (hpt) genes was delivered intoRobinia pseudoacacia L. callus using theAgrobacterium tumefaciens-mediated transformation method and particle gun transformation method. It was determined that adding 10 mg/L of acetosyringone
toAgrobacterium infection medium and callus-Agrobacterium co-culture medium could increaseAgrobacterium-mediated transformation efficiency. In contrast, particle gun transformation could successfully deliver plasmid DNA into
the callus, but its transformation efficiency was lower and gene expression was transient in comparison with theAgrobacterium-mediated method, suggesting that substantially stronger promoters might be required. |
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Keywords: | Agrobacterium tumefaciens callus culture particle gun Robinia pseudoacacia L Ti-plasmid transformation |
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