Recurrent selection using male sterility and hydroponic tiller culture in pedigree breeding of wheat |
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Authors: | G F Marais W C Botes J H Louw |
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Institution: | Department of Genetics, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa |
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Abstract: | A procedure for recurrent selection based on the male sterility gene, Ms3, was implemented. To facilitate the production of large numbers of hybrid progeny, a simple hydroponic system was developed in which male‐sterile tillers cut at the flowering stage can be pollinated and maintained for about 8 weeks‐long enough to produce a large quantity of viable hybrid seeds. The recurrent selection steps were integrated with a pedigree breeding programme employing different selection cycles for male and female plants. F1 female plants are subjected to a single screening for seedling resistance. In addition to F1 seedling screening, F2‐F4 male families are field‐selected for disease resistance, agrotype and quality in a pedigree programme before being used in crosses. |
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Keywords: | Triticum aestivum dominant male sterility Ms3 gene intercrossing recurrent selection |
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