| 牛肠道病毒的分离鉴定 |
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| 引用本文： | 李英利,常继涛,于力.牛肠道病毒的分离鉴定[J].中国预防兽医学报,2011,33(5). |
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| 作者姓名： | 李英利 常继涛 于力 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物病研究室,黑龙江,哈尔滨,150001 |
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| 摘 要： | 本研究从内蒙古某奶牛场的犊牛粪便样品中分离的一株牛肠道病毒(BEV),命名为BHM26,通过电镜观察显示和分子生物学鉴定,发现病毒粒子在细胞浆中呈结晶状排列,单一病毒粒子呈典型小RNA病毒粒子形态,直径为25nm～30nm,无囊膜;病毒的部分3D蛋白编码区和全长3'UTR区段的1 026bpDNA片段的RT-PCR扩增和序列分析表明,其核苷酸序列与GenBank登录的参考病毒株核苷酸序列同源性为71.9%～87%,其中与BEV Wye-3A株同源性最高,为87%.分析表明中国BEV分离株与国外参考毒株具有较大差异.
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| 关 键 词： | 牛肠道病毒 分离 鉴定 |
Isolation and identification of bovine enterovirus in China |
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| LI Ying-li,CHANG Ji-tao,YU Li.Isolation and identification of bovine enterovirus in China[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(5). |
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| Authors: | LI Ying-li CHANG Ji-tao YU Li |
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| Institution: | LI Ying-li,CHANG Ji-tao,YU Li (Division of Livestock Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | A bovine virus was isolated from the fecal samples of dairy cows/calves affected with diarrhea in China and identified as a bovine enterovirus (BEV) by RT-PCR and sequence analysis. The typical picornavirus particles were observed in infected cell by electron microscope. A conserved sequence including partial 3D protein encoding sequence and 3' UTR sequence (1,026 bp) of the isolate was amplified and sequenced. The phylogenetic analysis indicated that the isolate, designated BHM26, shared 71.9% to 87% nucle... |
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| Keywords: | bovine enterovirus isolation identification |
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