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| 重组截短型ELR1蛋白在昆虫表达系统中的分泌表达与结晶 | |
| 引用本文： | 孙佩龙,郝飞飞,吕淑霞,刘新奇,周建华,林跃智. 重组截短型ELR1蛋白在昆虫表达系统中的分泌表达与结晶[J]. 中国预防兽医学报, 2011, 33(6). DOI: 10.3969/j.issn.1008-0589.2011.06.04 |
| 作者姓名： | 孙佩龙 郝飞飞 吕淑霞 刘新奇 周建华 林跃智 |
| 作者单位： | 1. 沈阳农业大学生物科学技术学院,辽宁沈阳110161;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物病研究室,黑龙江哈尔滨150001 2. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物病研究室,黑龙江哈尔滨,150001 3. 沈阳农业大学生物科学技术学院,辽宁沈阳,110161 4. 南开大学生命科学学院,天津,300071 |
| 基金项目： | 国家自然科学青年基金，国家十一五重大传染病专项 |
| 摘 要： | 为获得马传染性贫血病毒单一受体(ELR1)的结晶体,本研究通过PCR方法获得ELR1胞外区基因片段,利用基因重组技术构建包含ELR1胞外区基因的重组杆状病毒表达质粒(Bacmid-ELR1).将其转染至昆虫细胞sf9中进行蛋白表达,western blot检测结果显示ELR1胞外区截短型重组蛋白在昆虫表达系统中获得表达.目的蛋白经亲和层析和凝胶过滤方法纯后化,通过多组结晶条件筛选获得了ELR1胞外区重组蛋白结晶体.本研究为ELR1立体结构和功能的解析提供了重要基础. |
| 关 键 词： | 马传染性贫血病毒单一受体 昆虫表达系统 纯化 结晶 |
The expression and crystallization of out-membrane region of equine lentiviral receptor 1 |
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| SUN Pei-long,HAO Fei-fei,LV Shu-xia,LIU Xin-qi,ZHOU Jian-hua,LIN yue-zhi. The expression and crystallization of out-membrane region of equine lentiviral receptor 1[J]. Chinese Journal of Preventive Veterinary Medicine, 2011, 33(6). DOI: 10.3969/j.issn.1008-0589.2011.06.04 | |
| Authors: | SUN Pei-long HAO Fei-fei LV Shu-xia LIU Xin-qi ZHOU Jian-hua LIN yue-zhi |
| Affiliation: | SUN Pei-long1,2#,HAO Fei-fei2#,LV Shu-xia1,LIU Xin-qi3,ZHOU Jian-hua2*,LIN yue-zhi2*(1.College of Biotechnology,Shenyang Agricultural University,Shenyang 110161,China,2.State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,3.Nankai University,Tianjin 300071,China) |
| Abstract: | The receptor for equine infectious anemia virus(EIAV) was identified as equine lentiviral receptor 1(ELR1) and classified as a member of the tumor necrosis factor receptor(TNFR) family according to its sequence characteristics.Differing from human,simian and feline immunodeficiency viruses,which need co-receptors for efficient infection,EIAV completes its infection by the solo ELR1.To understand the function of ELR1 and its interaction with EIAV,prokaryotic,eukaryotic expression and crystallization of out-m... |
| Keywords: | equine infectious anemia virus receptor eukaryotic expression purification crystallization |
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