| 鸡传染性支气管炎病毒ck/CH/LHB/100801株的分离鉴定及分子特征研究 |
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| 引用本文： | 马会杰,刘孝珍,韩宗玺,邵昱昊,孔宪刚,刘胜旺. 鸡传染性支气管炎病毒ck/CH/LHB/100801株的分离鉴定及分子特征研究[J]. 中国预防兽医学报, 2011, 33(5). DOI: 10.3969/j.issn.1008-0589.2011.05.04 |
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| 作者姓名： | 马会杰 刘孝珍 韩宗玺 邵昱昊 孔宪刚 刘胜旺 |
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| 作者单位： | 1. 东北农业大学,黑龙江,哈尔滨,150030;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨150001
2. 东北农业大学,黑龙江,哈尔滨,150030 |
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| 摘 要： | 本研究于2010年8月从河北省某鸡场鸡群中分离一株病毒,通过对分离株进行电子显微镜观察、鸡胚致病性试验、血凝特性试验等生物学特性及鸡传染性支气管炎病毒(IBV)核蛋白基因RT-PCR方法鉴定,结果表明分离株为IBV,命名为ck/CH/LHB/100801.根据GenBank中登录的IBV基因组序列,设计扩增病毒全基因组的19对引物,并扩增病毒基因组,采用3-RACE和5-RACE技术,扩增得到该病毒全部基因组序列.通过与已发表的参考病毒株全基因组序列比较表明,该分离株基因组全长为27 675bp,共有10个开放阅读框,其基因排序为:5'-cap-Replicase-S-3a-3b-3c-M-5a-5b-N-poly(A)-3',纤突蛋白裂解位点为534RRFRR538.序列分析表明ck/CH/LHB/100801与台湾分离株TW2296/95的结构蛋白序列相似性高达99.8%,进化亲缘关系最近,推测两分离株间可能具有相同的进化来源.
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| 关 键 词： | 鸡传染性支气管炎病毒 全基因序列 遗传进化分析 |
Molecular characteristics of avian infectious bronchitis virus ck/CH/LHB/100801 isolated in China |
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| MA Hui-jie,LIU Xiao-zhen,HAN Zong-xi,SHAO Yu-hao,KONG Xian-gang,LIU Sheng-wang. Molecular characteristics of avian infectious bronchitis virus ck/CH/LHB/100801 isolated in China[J]. Chinese Journal of Preventive Veterinary Medicine, 2011, 33(5). DOI: 10.3969/j.issn.1008-0589.2011.05.04 |
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| Authors: | MA Hui-jie LIU Xiao-zhen HAN Zong-xi SHAO Yu-hao KONG Xian-gang LIU Sheng-wang |
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| Affiliation: | MA Hui-jie1,2,LIU Xiao-zhen1,HAN Zong-xi1,SHAO Yu-hao1,KONG Xian-gang1,LIU Sheng-wang1 (1. Northeast Agricultural University,Harbin 150030,China,2. Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | An isolate of avian infectious bronchitis virus (IBV), designated ck/CH/LHB/100801), was isolated from Hebei area of China in 2010 and identified by electron microscope observation, pathogenicity to chicken embryo, hemagglutination test and RT-PCR. The complete genome was cloned by RT-PCR and sequenced including 3'-and 5'-terminals with 19 pairs of primers according to the genome sequences of IBV in GenBank. The sequences analysis showed that the 27,675 bp of ck/CH/LHB/100801 had the 5'-cap-Replicase-S-3a-3... |
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| Keywords: | infectious bronchitis virus the complete sequence phylogenetic analysis |
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